Marzucco Anna, Gatti Giulia, Montanari Maria Sofia, Fantini Michela, Colosimo Claudia, Tamburini Maria Vittoria, Arfilli Valentina, Morotti Manuela, Schiavone Pasqualina, Congestrì Francesco, Manera Martina, Denicolò Agnese, Brandolini Martina, Taddei Francesca, Grumiro Laura, Zannoli Silvia, Dirani Giorgio, De Pascali Alessandra Mistral, Sambri Vittorio, Cricca Monica
Unit of Microbiology, The Great Romagna Hub Laboratory, 47522 Pievesestina, Italy.
Department of Medical and Surgical Sciences-DIMEC, Alma Mater Studiorum-University of Bologna, 40126 Bologna, Italy.
Microorganisms. 2024 Jan 12;12(1):153. doi: 10.3390/microorganisms12010153.
spp. are an important opportunistic pathogen that can represent a possible cause of severe infections, especially in immunocompromised individuals. The clinical impact of spp. depends, in part, on the ability to form biofilms, communities of nestled cells into the extracellular matrix. In this study, we compared the biofilm formation ability of 83 strains of spp. isolated from blood cultures and other materials, such as respiratory samples, urine, and exudate, and their sensitivity to fluconazole (FLZ). Strains were divided into tertiles to establish cut-offs to classify isolates as low, moderate, or high biofilm producers (<0.26, 0.266-0.839, >0.839) and biofilms with low, moderate, or high metabolic activity (<0.053, 0.053-0.183, >0.183). A non-linear relationship between biofilm production and metabolic activity was found in and . In addition, the increase in minimum biofilm eradication concentrations (MBEC) compared to the Minor Inhibitory Concentration (PMIC) of the planktonic form in spp. confirms the role of biofilm in the induction of resistance to FLZ.
[该菌属]是一种重要的机会致病菌，可能是严重感染的一个潜在病因，尤其是在免疫功能低下的个体中。[该菌属]的临床影响部分取决于形成生物膜的能力，即细胞聚集在细胞外基质中形成群落的能力。在本研究中，我们比较了从血培养及其他样本（如呼吸道样本、尿液和渗出液）中分离出的83株[该菌属]菌株的生物膜形成能力及其对氟康唑（FLZ）的敏感性。菌株被分为三分位数以确定分类界限，将分离株分为低、中、高生物膜产生菌（<0.26、0.266 - 0.839、>0.839）以及具有低、中、高代谢活性的生物膜（<0.053、0.053 - 0.183、>0.183）。在[该菌属]中发现生物膜产生与代谢活性之间存在非线性关系。此外，[该菌属]中生物膜最低根除浓度（MBEC）相对于浮游形式的最低抑菌浓度（PMIC）的增加证实了生物膜在诱导对FLZ耐药性中的作用。
