Chen Tong, Zhang Shu, Yang Juan, Li Youran, Kogure Eiichi, Zhu Ye, Xiong Weiqi, Chen Enhui, Shi Guiyang
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
KAO (China) Research and Development Center, No. 623, Ziri Road, Minhang District, Shanghai 100098, China.
Microorganisms. 2024 Jan 13;12(1):160. doi: 10.3390/microorganisms12010160.
Washing machines are one of the tools that bring great convenience to people's daily lives. However, washing machines that have been used for a long time often develop issues such as odor and mold, which can pose health hazards to consumers. There exists a conspicuous gap in our understanding of the microorganisms that inhabit the inner workings of washing machines. In this study, samples were collected from 22 washing machines in Shanghai, China, including both water eluted from different parts of washing machines and biofilms. Quantitative qualitative analysis was performed using fluorescence PCR quantification, and microbial communities were characterized by high-throughput sequencing (HTS). This showed that the microbial communities in all samples were predominantly composed of bacteria. HTS results showed that in the eluted water samples, the bacteria mainly included , , , and . Conversely, in the biofilm samples, and were the predominant bacterial microorganisms. Correlation analysis results revealed that microbial colonies in washing machines were significantly correlated with years of use and the type of detergent used to clean the washing machine. As numerous pathogenic microorganisms can be observed in the results, effective preventive measures and future research are essential to mitigate these health problems and ensure the continued safe use of these household appliances.
洗衣机是给人们日常生活带来极大便利的工具之一。然而，长期使用的洗衣机常常会出现异味和霉菌等问题，这可能会对消费者的健康构成危害。我们对洗衣机内部存在的微生物的了解存在明显差距。在本研究中，从中国上海的22台洗衣机中采集了样本，包括从洗衣机不同部位洗脱的水和生物膜。使用荧光PCR定量进行了定量定性分析，并通过高通量测序（HTS）对微生物群落进行了表征。结果表明，所有样本中的微生物群落主要由细菌组成。HTS结果显示，在洗脱水样中，细菌主要包括 、 、 和 。相反，在生物膜样本中， 和 是主要的细菌微生物。相关性分析结果表明，洗衣机中的微生物菌落与使用年限以及用于清洁洗衣机的洗涤剂类型显著相关。由于在结果中可以观察到许多致病微生物，因此采取有效的预防措施和开展未来研究对于减轻这些健康问题并确保这些家用电器的持续安全使用至关重要。
