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表达 多阶段抗原的重组卡介苗疫苗为 BALB/c 小鼠提供了针对结核病的长期免疫力。

Recombinant BCG vaccine expressing multistage antigens of provides long-term immunity against tuberculosis in BALB/c mice.

机构信息

School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China.

National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

出版信息

Hum Vaccin Immunother. 2024 Dec 31;20(1):2299607. doi: 10.1080/21645515.2023.2299607. Epub 2024 Jan 23.

DOI:10.1080/21645515.2023.2299607
PMID:38258510
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10807470/
Abstract

Tuberculosis (TB) caused by (Mtb) persistently kills nearly 1.5 million lives per year in the world, whereas the only licensed TB vaccine BCG exhibits unsatisfactory efficacy in adults. Taking BCG as a vehicle to express Mtb antigens is a promising way to enhance its efficacy against Mtb infection. In this study, the immune efficacy of recombination BCG (rBCG-ECD003) expressing specific antigens ESAT-6, CFP-10, and nDnaK was evaluated at different time points after immunizing BALB/c mice. The results revealed that rBCG-ECD003 induced multiple Th1 cytokine secretion including IFN-γ, TNF-α, IL-2, and IL-12 when compared to the parental BCG. Under the action of PPD or ECD003, rBCG-ECD003 immunization resulted in a significant increase in the proportion of IL-2 and IFN-γIL-2 CD4T cells. Importantly, rBCG-ECD003 induced a stronger long-term humoral immune response without compromising the safety of the parental BCG vaccine. By means of the protective efficacy assay , rBCG-ECD003 showed a greater capacity to inhibit Mtb growth in the long term. Collectively, these features of rBCG-ECD003 indicate long-term protection and the promising effect of controlling Mtb infection.

摘要

结核病（TB）每年在全球造成近 150 万人死亡，而唯一获得许可的结核疫苗卡介苗（BCG）在成人中的疗效并不理想。以 BCG 作为载体表达结核分枝杆菌抗原是提高其对结核分枝杆菌感染疗效的一种有前途的方法。在这项研究中，在免疫 BALB/c 小鼠后的不同时间点评估了表达特定抗原 ESAT-6、CFP-10 和 nDnaK 的重组 BCG（rBCG-ECD003）的免疫效果。结果表明，与亲本 BCG 相比，rBCG-ECD003 诱导了多种 Th1 细胞因子的分泌，包括 IFN-γ、TNF-α、IL-2 和 IL-12。在 PPD 或 ECD003 的作用下，rBCG-ECD003 免疫导致 IL-2 和 IFN-γIL-2 CD4T 细胞的比例显著增加。重要的是，rBCG-ECD003 诱导了更强的长期体液免疫应答，而不会损害亲本 BCG 疫苗的安全性。通过保护效力测定，rBCG-ECD003 显示出在长期内抑制结核分枝杆菌生长的更大能力。总之，rBCG-ECD003 的这些特征表明了长期保护和控制结核分枝杆菌感染的有希望的效果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2da6/10807470/b993c30f2d0c/KHVI_A_2299607_F0007_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2da6/10807470/2b9775e0bc5d/KHVI_A_2299607_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2da6/10807470/6c4802bafbb0/KHVI_A_2299607_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2da6/10807470/8f38cd31d3e9/KHVI_A_2299607_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2da6/10807470/6b11059cc4f5/KHVI_A_2299607_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2da6/10807470/7256fe7316d8/KHVI_A_2299607_F0005_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2da6/10807470/ae3b852a8cc0/KHVI_A_2299607_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2da6/10807470/b993c30f2d0c/KHVI_A_2299607_F0007_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2da6/10807470/2b9775e0bc5d/KHVI_A_2299607_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2da6/10807470/6c4802bafbb0/KHVI_A_2299607_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2da6/10807470/8f38cd31d3e9/KHVI_A_2299607_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2da6/10807470/6b11059cc4f5/KHVI_A_2299607_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2da6/10807470/7256fe7316d8/KHVI_A_2299607_F0005_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2da6/10807470/ae3b852a8cc0/KHVI_A_2299607_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2da6/10807470/b993c30f2d0c/KHVI_A_2299607_F0007_OC.jpg

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[1]
Recombinant BCG vaccine expressing multistage antigens of provides long-term immunity against tuberculosis in BALB/c mice.

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[6]
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[7]
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[8]
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[9]
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[10]
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引用本文的文献

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Front Mol Biosci. 2025-8-8

[2]
Unleashing the power of the BCG vaccine in modulating viral immunity through heterologous protection: A scoping review.

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[3]
Development of a novel multi-epitope vaccine against Ureaplasma urealyticum infection through reverse vaccinology approach.

Mol Divers. 2025-6-19

[4]
The Role and Potential Application of IL-12 in the Immune Regulation of Tuberculosis.

Int J Mol Sci. 2025-3-28

本文引用的文献

[1]
CD8+ lymphocytes are critical for early control of tuberculosis in macaques.

J Exp Med. 2023-12-4

[2]
A Key Role of CD8+ T Cells in Controlling of Tuberculosis Infection.

Diagnostics (Basel). 2023-9-15

[3]
Immunogenicity and efficacy analyses of EPC002, ECA006, and EPCP009 protein subunit combinations as tuberculosis vaccine candidates.

Vaccine. 2023-6-13

[4]
A novel multi-component protein vaccine ECP001 containing a protein polypeptide antigen nPstS1 riching in T-cell epitopes showed good immunogenicity and protection in mice.

Front Immunol. 2023

[5]
The Induction of Antigen 85B-Specific CD8 T Cells by Recombinant BCG Protects against Mycobacterial Infection in Mice.

Int J Mol Sci. 2023-1-4

[6]
Construction and immunogenicity of a T cell epitope-based subunit vaccine candidate against Mycobacterium tuberculosis.

Vaccine. 2021-11-16

[7]
The direct mycobacterial growth inhibition assay (MGIA) for the early evaluation of TB vaccine candidates and assessment of protective immunity: a protocol for non-human primate cells.

F1000Res. 2021

[8]
One-Year Old Dormant, "Non-culturable" Preserves Significantly Diverse Protein Profile.

Front Cell Infect Microbiol. 2020

[9]
Adaption of the ex vivo mycobacterial growth inhibition assay for use with murine lung cells.

Sci Rep. 2020-2-24

[10]
Final Analysis of a Trial of M72/AS01 Vaccine to Prevent Tuberculosis.

N Engl J Med. 2019-10-29

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