Kudryavtsev Igor, Zinchenko Yulia, Serebriakova Maria, Akisheva Tatiana, Rubinstein Artem, Savchenko Andrei, Borisov Alexandr, Belenjuk Vasilij, Malkova Anna, Yablonskiy Piotr, Kudlay Dmitry, Starshinova Anna
Institution of Experimental Medicine, Department of Immunology, 197376 St-Petersburg, Russia.
Almazov National Medical Research Centre, 197341 St-Petersburg, Russia.
Diagnostics (Basel). 2023 Sep 15;13(18):2961. doi: 10.3390/diagnostics13182961.
The main role in the control of tuberculosis infection is played by macrophages and Th1 and CD8+ T cells. The study aimed to identify the most diagnostically significant CD8+ T cell subsets in tuberculosis patients.
Peripheral blood samples from patients with clinical, radiological, and bacteriologically confirmed pulmonary tuberculosis (TB, n = 32) and healthy subjects (HC, n = 31) were collected and analyzed using 10-color flow cytometry.
The frequency of the EM4 CD3+CD8+ cells was reduced in the peripheral blood of patients with pulmonary tuberculosis, while the relative and absolute number of EM1 CD3+CD8+ cells increased compared to the control group. CD57 expression was reduced in patients with pulmonary tuberculosis on EM1, EM2, and pE1 CD3+CD8+ cells, whereas the EM3 cells had a high level of CD57 expression. The relative and absolute number of Tc2 (CCR6-CXCR3-) cells in peripheral blood in patients with pulmonary tuberculosis was increased, while the frequency of Tc1 (CCR6-CXCR3+) was decreased, compared to healthy donors.
Patients with pulmonary tuberculosis have an abnormal CD3+CD8+ cell profile and demonstrate their impaired maturation and functional activity.
巨噬细胞、Th1和CD8 + T细胞在结核病感染控制中起主要作用。本研究旨在确定结核病患者中最具诊断意义的CD8 + T细胞亚群。
收集临床、放射学和细菌学确诊的肺结核患者（TB，n = 32）和健康受试者（HC，n = 31）的外周血样本，采用10色流式细胞术进行分析。
肺结核患者外周血中EM4 CD3 + CD8 +细胞频率降低，而与对照组相比，EM1 CD3 + CD8 +细胞的相对和绝对数量增加。肺结核患者EM1、EM2和pE1 CD3 + CD8 +细胞上的CD57表达降低，而EM3细胞的CD57表达水平较高。与健康供体相比，肺结核患者外周血中Tc2（CCR6 - CXCR3 -）细胞的相对和绝对数量增加，而Tc1（CCR6 - CXCR3 +）的频率降低。
肺结核患者的CD3 + CD8 +细胞谱异常，表明其成熟和功能活性受损。
