Xu Linglan, Xie Nan, Liu Yiqin, Tang Hongmei, He Jinjiang, He Zhen, Zheng Kang, Li Ranhui
Hunan Province Clinical Medical Research Center for Occupational Diseases, Hunan Prevention and Treatment Institute for Occupational Diseases, Affiliated Prevention and Treatment Institute for Occupational Diseases of University of South China, University of South China, Changsha, 410007, China.
Affiliated Hengyang Hospital of Hunan Normal University & Hengyang Central Hospital, Hengyang, 421001, Hunan, China.
Mol Divers. 2025 Jun 19. doi: 10.1007/s11030-025-11234-2.
Ureaplasma urealyticum (U. urealyticum) is a sexually transmitted pathogen often causing urogenital tract disorders. The growing challenge of multidrug-resistant strains poses a significant risk for the treatment of U. urealyticum infections. To date, no licensed vaccines are available, and previous attempts to create secure and efficient prophylaxis have been failed. Recent studies have adopted an immunoinformatic strategy based on reverse vaccinology to detect antigenic proteins which are appropriate for the creation of a multi-epitope vaccine. The multi-epitope subunit vaccine, incorporating eleven T-cell and seven B-cell epitopes along with the adjuvant, exhibited strong antigenicity and did not induce allergic responses. Moreover, molecular docking as well as dynamic simulations were utilized to investigate the interaction within the vaccine-adjuvant complex. The prospective effectiveness of the vaccine was verified via immune simulation experiments. Therefore, the vaccine developed in this study represents an effective multi-epitope solution for immunization against U. urealyticum, waiting for further experimental analysis.
解脲脲原体是一种常引起泌尿生殖道疾病的性传播病原体。多重耐药菌株带来的挑战日益增加，给解脲脲原体感染的治疗带来了重大风险。迄今为止，尚无获批的疫苗，以往创建安全有效的预防措施的尝试均告失败。最近的研究采用了基于反向疫苗学的免疫信息学策略来检测适合创建多表位疫苗的抗原蛋白。包含11个T细胞和7个B细胞表位以及佐剂的多表位亚单位疫苗表现出很强的抗原性，且不诱导过敏反应。此外，利用分子对接和动力学模拟来研究疫苗-佐剂复合物内部的相互作用。通过免疫模拟实验验证了该疫苗的预期有效性。因此，本研究开发的疫苗代表了一种针对解脲脲原体免疫的有效多表位解决方案，有待进一步的实验分析。
