Key Contributions of the Overexpressed Sigma Glutathione -Transferase 1 Gene () in the Resistance Evolution to Multiple Insecticides.

The overexpression of insect detoxification enzymes is a typical adaptive evolutionary strategy for insects to cope with insecticide pressure. In this study, we identified a glutathione -transferase (GST) gene, , that exhibited pronounced expression in the field-resistant population of . By using RNAi (RNA interference), the transgenic fly models, and quantitative real-time polymerase chain reaction (RT-qPCR) methods, we confirmed that the augmented expression of mediates the resistance of to various types of insecticides, including chlorantraniliprole, novaluron, λ-cyhalothrin, and abamectin. was found to bolster insecticide resistance in two ways: direct detoxification and enhancing antioxidative defenses. In addition, our findings demonstrated that pxy-miR-8528a exerts a pivotal influence on forming insecticide resistance in by downregulating expression. In summary, we elucidated the multifaceted molecular and biochemical underpinnings of -driven insecticide resistance in . Our results provide a new perspective for understanding the insecticide resistance mechanism of .