Zolfaghari Maryam, Yin Fei, Jurat-Fuentes Juan Luis, Xiao Yong, Peng Zhengke, Wang Jiale, Yang Xiangbing, Li Zhen-Yu
Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Insects. 2024 Aug 5;15(8):595. doi: 10.3390/insects15080595.
Detoxification genes are crucial to insect resistance against chemical pesticides, yet their expression may be altered by exposure to biopesticides such as spores and insecticidal proteins of (Bt). Increased enzymatic levels of selected detoxification genes, including glutathione S-transferase (GST), cytochrome P450 (CYP450), and carboxylesterase (CarE), were detected in chlorantraniliprole (CAP)-resistant strains of the diamondback moth (DBM, ) from China when compared to a reference susceptible strain. These CAP-resistant DBM strains displayed distinct expression patterns of GST 1, CYP6B7, and CarE-6 after treatment with CAP and a Bt pesticide (Bt-G033). In particular, the gene expression analysis demonstrated significant upregulation of the CYP6B7 gene in response to the CAP treatment, while the same gene was downregulated following the Bt-G033 treatment. Downregulation of CYP6B7 using RNAi resulted in increased susceptibility to CAP in resistant DBM strains, suggesting a role of this gene in the resistant phenotype. However, pretreatment with a sublethal dose of Bt-G033 inducing the downregulation of CYP6B7 did not significantly increase CAP potency against the resistant DBM strains. These results identify the DBM genes involved in the metabolic resistance to CAP and demonstrate how their expression is affected by exposure to Bt-G033.
解毒基因对于昆虫抵抗化学农药至关重要，然而其表达可能会因接触生物农药（如苏云金芽孢杆菌（Bt）的孢子和杀虫蛋白）而发生改变。与参考敏感品系相比，在中国小菜蛾（DBM，Plutella xylostella）的氯虫苯甲酰胺（CAP）抗性品系中检测到了所选解毒基因（包括谷胱甘肽S-转移酶（GST）、细胞色素P450（CYP450）和羧酸酯酶（CarE））的酶水平升高。这些对CAP具有抗性的小菜蛾品系在用CAP和一种Bt农药（Bt-G033）处理后，显示出GST 1、CYP6B7和CarE-6的不同表达模式。特别是，基因表达分析表明，CYP6B7基因在CAP处理后显著上调，而在Bt-G033处理后该基因下调。使用RNA干扰下调CYP6B7导致抗性小菜蛾品系对CAP的敏感性增加，表明该基因在抗性表型中起作用。然而，用亚致死剂量的Bt-G033预处理诱导CYP6B7下调，并没有显著提高CAP对抗性小菜蛾品系的效力。这些结果确定了参与对CAP代谢抗性的小菜蛾基因，并证明了它们的表达如何受到Bt-G033暴露的影响。
