MOA Key Laboratory of Pest Monitoring and Green Management, Department of Entomology, College of Plant Protection, China Agricultural University, 2 Yuanmingyuan West Road, Beijing 100193, China.
Institute of Molecular Sciences and Engineering, Institute of Frontier and Interdisciplinary Science, Shandong University, Qingdao 266237, China.
J Agric Food Chem. 2022 May 18;70(19):5794-5804. doi: 10.1021/acs.jafc.2c01867. Epub 2022 May 5.
The diamondback moth, (L.), has evolved with varying degrees of resistance to almost all major classes of insecticides and has become the most resistant pest worldwide. The multiresistance to different types of insecticides has been frequently reported in , but little is known about the mechanism. In this study, a carboxylesterase (CarE) gene, , was found significantly overexpressed in a field-evolved multiresistant population and can be dramatically induced by eight of nine tested insecticides. Results of the real-time quantitative polymerase chain reaction (RT-qPCR) showed that was predominantly expressed in the midgut and malpighian tubule of larvae. Knockdown of dramatically increased the susceptibility of the larvae to β-cypermethrin, bifenthrin, chlorpyrifos, fenvalerate, malathion, and phoxim, while overexpression of in increased the tolerance of the fruit flies to these insecticides obviously. More importantly, gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay showed that the recombinant PxαE14 expressed in exhibited metabolic activity against the six insecticides. The homology modeling, molecular docking, and molecular dynamics simulation analyses showed that these six insecticides could stably bind to PxαE14. Taken together, these results demonstrate that constitutive and inductive overexpression of contributes to detoxification of multiple insecticides involved in multiresistance in . Our findings provide evidence for understanding the molecular mechanisms underlying the multiresistance in insect pests.
美洲大蠊，（L.），已进化出对几乎所有主要类别的杀虫剂的不同程度的抗性，并已成为世界上最具抗性的害虫。在 中，经常报道对不同类型杀虫剂的多抗性，但对其机制知之甚少。在这项研究中，发现一种羧酸酯酶（CarE）基因，，在田间进化的多抗性 种群中显著过表达，并且可以被测试的九种杀虫剂中的八种显著诱导。实时定量聚合酶链反应（RT-qPCR）的结果表明，在幼虫的中肠和马氏管中主要表达。 基因的敲低显著增加了幼虫对β-氯氰菊酯、联苯菊酯、毒死蜱、氰戊菊酯、马拉硫磷和辛硫磷的敏感性，而 在 中的过表达明显增加了果蝇对这些杀虫剂的耐受性。更重要的是，气相色谱-质谱联用（GC-MS）和液相色谱-串联质谱联用（LC-MS/MS）检测表明，在 中表达的重组 PxαE14 对六种杀虫剂具有代谢活性。同源建模、分子对接和分子动力学模拟分析表明，这六种杀虫剂可以稳定地与 PxαE14 结合。总之，这些结果表明，的组成型和诱导性过表达有助于参与多抗性的多种杀虫剂的解毒。我们的研究结果为理解昆虫多抗性的分子机制提供了证据。
