Nanobiosensors and Bioanalytical Applications Group (NanoB2A), Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, CIBER-BBN and BIST, 08193, Bellaterra, Barcelona, Spain.
Nanobiosensors and Bioanalytical Applications Group (NanoB2A), Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC, CIBER-BBN and BIST, 08193, Bellaterra, Barcelona, Spain.
Talanta. 2024 May 1;271:125685. doi: 10.1016/j.talanta.2024.125685. Epub 2024 Jan 16.
The coronavirus disease 2019 (COVID-19) pandemic recently demonstrated the devastating impact on public health, economy, and social development of zoonotic infectious diseases, whereby viruses jump from animals to infect humans. Due to this potential of viruses to cross the species barrier, the surveillance of infectious pathogens circulation in domestic and close-to-human animals is indispensable, as they could be potential reservoirs. Optical biosensors, mainly those based on Surface Plasmon Resonance (SPR), have widely demonstrated its ability for providing direct, label-free, and quantitative bioanalysis with excellent sensitivity and reliability. This biosensor technology can provide a powerful tool to the veterinary field, potentially being helpful for the monitoring of the infection spread. We have implemented a multi-target COVID-19 serology plasmonic biosensor for the rapid testing and screening of common European domestic animals. The multi-target serological biosensor assay enables the detection of total SARS-CoV-2 antibodies (IgG + IgM) generated towards both S and N viral antigens. The analysis is performed in less than 15 min with a low-volume serum sample (<20 μL, 1:10 dilution), reaching a limit of detection of 49.6 ng mL. A complete validation has been carried out with hamster, dog, and cat sera samples (N = 75, including 37 COVID-19-positive and 38 negative samples). The biosensor exhibits an excellent diagnostic sensitivity (100 %) and good specificity (71.4 %) for future application in veterinary settings. Furthermore, the biosensor technology is integrated into a compact, portable, and user-friendly device, well-suited for point-of-care testing. This study positions our plasmonic biosensor as an alternative and reliable diagnostic tool for COVID-19 serology in animal samples, expanding the applicability of plasmonic technologies for decentralized analysis in veterinary healthcare and animal research.
2019 年冠状病毒病（COVID-19）大流行最近表明，人畜共患传染病对公共卫生、经济和社会发展造成了破坏性影响，病毒由此从动物传播到人类。由于病毒有跨越物种屏障的潜在能力，对人畜传染病的病原体循环进行监测是必不可少的，因为它们可能是潜在的宿主。光学生物传感器，主要是基于表面等离子体共振（SPR）的生物传感器，已经广泛证明了其提供直接、无标记和定量生物分析的能力，具有出色的灵敏度和可靠性。这项生物传感器技术可以为兽医领域提供一个强大的工具，可能有助于监测感染的传播。我们已经开发了一种用于快速检测和筛查常见欧洲家养动物的多靶标 COVID-19 血清学等离子体生物传感器。该多靶标血清学生物传感器分析能够检测针对 S 和 N 病毒抗原产生的总 SARS-CoV-2 抗体（IgG+IgM）。分析在不到 15 分钟的时间内完成，使用小体积血清样本（<20 μL，1:10 稀释），检测限达到 49.6 ng mL。我们对仓鼠、狗和猫血清样本（N=75，包括 37 个 COVID-19 阳性样本和 38 个阴性样本）进行了全面验证。该生物传感器对未来在兽医环境中的应用表现出出色的诊断灵敏度（100%）和良好的特异性（71.4%）。此外，该生物传感器技术集成到一个紧凑、便携和用户友好的设备中，非常适合现场即时检测。本研究将我们的等离子体生物传感器定位为动物样本 COVID-19 血清学的替代和可靠诊断工具，扩展了等离子体技术在兽医保健和动物研究中用于分散式分析的适用性。
