Wolfe R R, Peters E J
Am J Physiol. 1987 Feb;252(2 Pt 1):E218-23. doi: 10.1152/ajpendo.1987.252.2.E218.
We have determined the effect of various rates of glucose infusion on the rates of release of glycerol (Ra glycerol), free fatty acids (Ra FFA), and on energy metabolism in normal human volunteers. Plasma kinetics were determined with use of the stable isotopic tracers D-5-glycerol and [1-13C]palmitate, and energy metabolism was determined by indirect calorimetry. The effect of glucose infusion on Ra glycerol and Ra FFA was dose-dependent. At an infusion rate of 1 mg X kg-1 X min-1, no effect on either kinetic factor was noted. At 4 mg X kg-1 X min-1, both Ra glycerol and Ra FFA were suppressed; at 8 mg X kg-1 X min-1, Ra FFA was even more depressed, but Ra glycerol was similar to the value during the 4 mg X kg-1 X min-1 infusion. At all infusion rates tested, the amount of potential energy available from the sum of the glucose infusion and endogenously mobilized fat was always greater than when no glucose was infused. Glucose decreased fat mobilization by both inhibiting lipolysis and stimulating reesterification, thus causing a significant increase in triglyceride-fatty acid substrate cycling within the adipose tissue.
我们已经确定了不同葡萄糖输注速率对正常人类志愿者甘油（甘油释放率，Ra甘油）、游离脂肪酸（Ra游离脂肪酸）释放率以及能量代谢的影响。使用稳定同位素示踪剂D-5-甘油和[1-13C]棕榈酸酯测定血浆动力学，并通过间接量热法测定能量代谢。葡萄糖输注对Ra甘油和Ra游离脂肪酸的影响呈剂量依赖性。在输注速率为1mg·kg-1·min-1时，未观察到对任何一个动力学因素有影响。在4mg·kg-1·min-1时，Ra甘油和Ra游离脂肪酸均受到抑制；在8mg·kg-1·min-1时，Ra游离脂肪酸受到的抑制更明显，但Ra甘油与4mg·kg-1·min-1输注期间的值相似。在所有测试的输注速率下，葡萄糖输注和内源性动员脂肪的总和所提供的潜在能量总是大于未输注葡萄糖时。葡萄糖通过抑制脂肪分解和刺激再酯化来减少脂肪动员，从而导致脂肪组织内甘油三酯-脂肪酸底物循环显著增加。
