State Key Laboratory of Enhanced Oil Recovery, PetroChina Research Institute of Petroleum Exploration and Development, Beijing 100083, China.
State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210023, China.
Org Lett. 2024 Feb 2;26(4):971-976. doi: 10.1021/acs.orglett.4c00052. Epub 2024 Jan 24.
Cinnamoyl-containing nonribosomal peptides (CCNPs) constitute a unique family of actinobacterial secondary metabolites that display a broad spectrum of biological activities. Here, we present a genome mining approach targeting cyclase and is isomerase to discover new CCNPs, which led to the identification of 207 putative CCNP gene clusters from public bacterial genome databases. After strain prioritization, a novel class of CCNP-type glycopeptides named malacinnamycin was identified. A plausible biosynthetic pathway for malacinnamycin was deduced by bioinformatics analysis.
含肉桂酰基的非核糖体肽（CCNPs）构成了放线菌次级代谢产物的一个独特家族，具有广泛的生物活性。在这里，我们提出了一种针对环化酶和异构酶的基因组挖掘方法，以发现新的 CCNPs，从而从公共细菌基因组数据库中鉴定出 207 个推定的 CCNP 基因簇。经过菌株优先级排序，鉴定出一种新型的 CCNP 型糖肽，命名为马拉西那霉素。通过生物信息学分析推断出马拉西那霉素的可能生物合成途径。
