Department of Cardiology, The Third Affiliated Hospital of Soochow University, Changzhou 213003, China.
Clinical Medical Research Center, The Third Affiliated Hospital of Soochow University, Changzhou 213003, China.
Neurobiol Dis. 2024 Mar;192:106415. doi: 10.1016/j.nbd.2024.106415. Epub 2024 Jan 22.
The prevalence of depression is higher in heart failure (HF) patients. Early screening of depressive symptoms in HF patients and timely intervention can help to improve patients' quality of life and prognosis. This study aims to explore diagnostic biomarkers by examining the expression profile of serum exosomal miRNAs in HF patients with depressive symptoms.
Serum exosomal RNA was isolated and extracted from 6 HF patients with depressive symptoms (HF-DS) and 6 HF patients without depressive symptoms (HF-NDS). High-throughput sequencing was performed to obtain miRNA expression profiles and target genes were predicted for the screened differentially expressed miRNAs. Biological functions of the target genes were analyzed through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Subsequently, we collected serum exosomal RNAs from HF-DS (n = 20) and HF-NDS (n = 20). The differentially expressed miRNAs selected from the sequencing results were validated using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Finally, the diagnostic efficacy of the differentially expressed exosomal miRNAs for HF-DS was evaluated by using receiver operating characteristic (ROC) curves.
A total of 19 significantly differentially expressed exosomal miRNAs were screened by high-throughput sequencing, consisting of 12 up-regulated and 7 down-regulated exosomal miRNAs. RT-qPCR validation demonstrated that the expression level of exo-miR-144-3p was significantly down-regulated in the HF-DS group, and the expression levels of exo-miR-625-3p and exo-miR-7856-5p were significantly up-regulated. In addition, the expression level of exo-miR-144-3p was negatively correlated with the severity of depressive symptoms in HF patients, and that the area under the curve (AUC) of exo-miR-144-3p for diagnosing HF-DS was 0.763.
In this study, we examined the serum exosomal miRNA expression profiles of HF patients with depressive symptoms and found that lower level of exo-miR-144-3p was associated with more severe depressive symptoms. Exo-miR-144-3p is a potential biomarker for the diagnosis of HF-DS.
心力衰竭（HF）患者的抑郁患病率较高。早期筛查 HF 患者的抑郁症状并及时干预，有助于改善患者的生活质量和预后。本研究旨在通过检测 HF 伴抑郁症状（HF-DS）患者血清外泌体 miRNA 的表达谱，探讨诊断生物标志物。
从 6 例 HF-DS 患者和 6 例 HF 无抑郁症状（HF-NDS）患者的血清中分离和提取血清外泌体 RNA。进行高通量测序以获得 miRNA 表达谱，并对筛选出的差异表达 miRNA 进行靶基因预测。通过基因本体（GO）和京都基因与基因组百科全书（KEGG）分析靶基因的生物学功能。随后，我们收集了 HF-DS（n=20）和 HF-NDS（n=20）患者的血清外泌体 RNA。使用逆转录定量聚合酶链反应（RT-qPCR）验证测序结果中选择的差异表达 miRNA。最后，通过受试者工作特征（ROC）曲线评估差异表达外泌体 miRNA 对 HF-DS 的诊断效能。
高通量测序共筛选出 19 个差异表达外泌体 miRNA，包括 12 个上调和 7 个下调的外泌体 miRNA。RT-qPCR 验证显示，HF-DS 组外泌体 miR-144-3p 的表达水平显著下调，外泌体 miR-625-3p 和外泌体 miR-7856-5p 的表达水平显著上调。此外，外泌体 miR-144-3p 的表达水平与 HF 患者抑郁症状的严重程度呈负相关，外泌体 miR-144-3p 诊断 HF-DS 的曲线下面积（AUC）为 0.763。
本研究检测了 HF 伴抑郁症状患者的血清外泌体 miRNA 表达谱，发现外泌体 miR-144-3p 水平较低与更严重的抑郁症状相关。外泌体 miR-144-3p 是 HF-DS 诊断的潜在生物标志物。
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