Yul Lee Hee, Haque Md Azizul, Yong Cho Du, Bin Jeong Jong, Ho Lee Ji, Young Lee Ga, Yeun Jang Mu, Hwan Lee Jin, Man Cho Kye
Department of GreenBio Science and Agri-Food Bio Convergence Institute, Gyeongsang National University, Jinju 52727, Republic of Korea.
Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Science and Technology University, Dinajpur 5200, Bangladesh.
Food Chem X. 2023 Dec 25;21:101101. doi: 10.1016/j.fochx.2023.101101. eCollection 2024 Mar 30.
In this study, the microbial diversity, free amino acid (FAA), and biological activities of household (HDJ) from four different regions and commercial (CDJ) four manufacturers were analyzed. And volatile flavor compound (VFC) and isoflavone profiles were analyzed using gas chromatograph-mass spectrometer (GC-MS) and high performance liquid chromatography (HPLC), respectively. The major bacterial genus in 1HDJ and 2HDJ was (97.5%), while in 3HDJ and 4HDJ, it was (47.5%) and (80%), respectively. was the main bacterial genus of CDJ. The genus among yeast was comparatively high in all samples. In all samples, glutamic acid predominated among the FAAs, and the 3-methyl butanal, benzeneacetaldehyde, and diallyl disulphide were major VFCs. CDJ contained higher levels of isoflavone-glycoside and total phenolics. Except for 3HDJ and 4CDJ, the isoflavone-aglycone and total flavonoid contents were higher in HDJ. The correlation between bacterial genus and metabolited of showed that was closely related to glutamic acid, was related to aglycones and ammonia, and was highly related to isovaleric acid. While, correlation between yeast genus and metabolited of confirmed that , , and were related with furfural, benzeneacetaldehyde, and 3-methyl butanal, respectively. The results of this study can be utilized as basic data for the industrialization and development of .
在本研究中,分析了来自四个不同地区的家庭酿造酱油(HDJ)和四个制造商的市售酱油(CDJ)的微生物多样性、游离氨基酸(FAA)和生物活性。分别使用气相色谱 - 质谱联用仪(GC - MS)和高效液相色谱仪(HPLC)分析了挥发性风味化合物(VFC)和异黄酮谱。1HDJ和2HDJ中的主要细菌属为(97.5%),而在3HDJ和4HDJ中,分别为(47.5%)和(80%)。是CDJ的主要细菌属。酵母中的属在所有样品中相对较高。在所有样品中,FAA中谷氨酸占主导地位,3 - 甲基丁醛、苯乙醛和二烯丙基二硫化物是主要的VFC。CDJ含有较高水平的异黄酮糖苷和总酚类。除了3HDJ和4CDJ外,HDJ中的异黄酮苷元和总黄酮含量较高。细菌属与代谢产物之间的相关性表明,与谷氨酸密切相关,与苷元和氨有关,与异戊酸高度相关。同时,酵母属与代谢产物之间的相关性证实,分别与糠醛、苯乙醛和3 - 甲基丁醛有关。本研究结果可作为工业化和发展的基础数据。