Shen Wenzheng, Fu Yu, Bai Haiyu, Zhang Zhiyu, Cao Zhikun, Liu Zibo, Yang Chao, Sun Shixin, Wang Lei, Ren Chunhuan, Ling Yinghui, Zhang Zijun, Cao Hongguo
College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Anhui Agricultural University, Hefei, 230036, China.
Anim Biosci. 2024 Apr;37(4):640-654. doi: 10.5713/ab.23.0329. Epub 2024 Jan 20.
The purpose of this study was to explore the effect of sodium salicylate (SS) on semen preservation and metabolic regulation in goats.
Under the condition of low temperature, SS was added to goat semen diluent to detect goat sperm motility, plasma membrane, acrosome, antioxidant capacity, mitochondrial membrane potential (MMP) and metabonomics.
The results show that at the 8th day of low-temperature storage, the sperm motility of the 20 μM SS group was 66.64%, and the integrity rates of the plasma membrane and acrosome were both above 60%, significantly higher than those of the other groups. The activities of catalase and superoxide dismutase in the sperm of the 20 μM SS group were significantly higher than those of the control group, the contents of reactive oxygen species and malondialdehyde were significantly lower than those in the control group, the MMP was significantly higher than that in the control group, and the contents of Ca2+ and total cholesterol were significantly higher than those in the control group. Through metabonomics analysis, there were significant metabolic differences between the control group and the 20 μM SS group. Twenty of the most significant metabolic markers were screened, mainly involving five metabolic pathways, of which nicotinic acid and nicotinamide metabolic pathways were the most significant.
The results indicate that SS can effectively improve the low-temperature preservation quality of goat sperm.
本研究旨在探讨水杨酸钠（SS）对山羊精液保存及代谢调节的影响。
在低温条件下，将SS添加到山羊精液稀释液中，检测山羊精子活力、质膜、顶体、抗氧化能力、线粒体膜电位（MMP）和代谢组学。
结果显示，在低温保存第8天时，20 μM SS组精子活力为66.64%，质膜和顶体完整率均高于60%，显著高于其他组。20 μM SS组精子中过氧化氢酶和超氧化物歧化酶活性显著高于对照组，活性氧和丙二醛含量显著低于对照组，MMP显著高于对照组，Ca2+和总胆固醇含量显著高于对照组。通过代谢组学分析，对照组和20 μM SS组之间存在显著的代谢差异。筛选出20个最显著的代谢标志物，主要涉及五条代谢途径，其中烟酸和烟酰胺代谢途径最为显著。
结果表明，SS能有效提高山羊精子的低温保存质量。
