Maehara Y, Miyamoto K, Anai H, Kusumoto H, Fukuchi K, Masuda H, Sugimachi K
Gan To Kagaku Ryoho. 1987 Mar;14(3 Pt 1):630-4.
The predicted level of cell viability was compared between the succinate dehydrogenase inhibition (SDI) test and the adenosine triphosphate (ATP) assay, both of which are used for in vitro human tumor chemosensitivity testing. After HeLa cells had been exposed to various concentrations of 5-FU for 1, 2, 3 or 4 days, the decrease occurring in viable cell number correlated with that of succinate dehydrogenase (EC 1.3.99.1) activity and that of the intracellular ATP level of the viable cells. In dead cells, the ATP level was extensively decreased, but the succinate dehydrogenase activity remained at a level of 11% of that of 5-FU-untreated viable cells, even on day 4. The cell viability correlated well with the intracellular ATP level, as compared with the succinate dehydrogenase activity. The activity remaining in dead cells must thus be taken into consideration for the prediction of chemosensitivity in the SDI test, but not in the ATP assay.
在用于体外人肿瘤化学敏感性测试的琥珀酸脱氢酶抑制（SDI）试验和三磷酸腺苷（ATP）检测之间，对预测的细胞活力水平进行了比较。将HeLa细胞暴露于不同浓度的5-氟尿嘧啶（5-FU）1、2、3或4天后，存活细胞数量的减少与琥珀酸脱氢酶（EC 1.3.99.1）活性以及存活细胞的细胞内ATP水平的降低相关。在死亡细胞中，ATP水平大幅下降，但即使在第4天，琥珀酸脱氢酶活性仍保持在未用5-FU处理的存活细胞活性的11%。与琥珀酸脱氢酶活性相比，细胞活力与细胞内ATP水平密切相关。因此，在SDI试验中预测化学敏感性时必须考虑死亡细胞中残留的活性，但在ATP检测中则无需考虑。
