Tani Naoto, Ikeda Tomoya, Ishikawa Takaki
Department of Legal Medicine, Graduate School of Medicine, Osaka Metropolitan University, 1-4-3 Asahi-machi, Abeno, Osaka 545-8585, Japan.
Forensic Autopsy Section, Medico-Legal Consultation and Postmortem Investigation Support Center, 1-4-3 Asahi-machi, Abeno, Osaka 545-8585, Japan.
Life (Basel). 2024 Jan 21;14(1):152. doi: 10.3390/life14010152.
The levels and potential role of prolactin (PRL) in the brain under conditions of acute systemic hypoxia were examined, focusing on the accumulation of PRL in cerebrospinal fluid (CSF) and its effects on neuronal activity and injury. The amount of PRL in the brain was investigated using brain tissues from forensic autopsy cases. We counted the number of neurites that formed in human primary neurons (HNs) after the addition of PRL. Furthermore, HNs supplemented with PRL or triiodothyronine (T3) were exposed to hypoxic conditions, and the dead cells were counted. The results showed correlations between brain PRL and CSF PRL levels. Additionally, PRL accumulation in the brain was observed in cases of asphyxia. In vitro experimental findings indicated increased neurite formation in the HNs treated with PRL. Moreover, both PRL and T3 demonstrated neuroprotective effects against hypoxia-induced neuronal cell death, with PRL showing stronger neuroprotective potential than T3. These results suggest that PRL accumulates in the brain during hypoxia, potentially influences neuronal activity, and exhibits neuroprotective properties against hypoxia-induced neuronal injury.
研究了急性全身性缺氧条件下大脑中催乳素（PRL）的水平及其潜在作用，重点关注脑脊液（CSF）中PRL的积累及其对神经元活性和损伤的影响。使用法医尸检病例的脑组织研究大脑中PRL的含量。我们计算了添加PRL后人原代神经元（HN）中形成的神经突数量。此外，将补充有PRL或三碘甲状腺原氨酸（T3）的HN暴露于缺氧条件下，并对死亡细胞进行计数。结果显示大脑PRL与脑脊液PRL水平之间存在相关性。此外，在窒息病例中观察到大脑中PRL的积累。体外实验结果表明，用PRL处理的HN中神经突形成增加。此外，PRL和T3均对缺氧诱导的神经元细胞死亡具有神经保护作用，PRL的神经保护潜力比T3更强。这些结果表明，PRL在缺氧期间在大脑中积累，可能影响神经元活性，并对缺氧诱导的神经元损伤具有神经保护特性。
