Turkoglu Fatih, Calisir Akin, Ozturk Bahadir
Department of General Surgery, Faculty of Medicine, Selcuk University, Akademi Mahallesi Yeni İstanbul Caddesi No:313, Selçuk Üniversitesi Alaeddin Keykubat Yerleşkesi, Selçuklu, Konya, 42130, Turkey.
Department of Biochemistry, Faculty of Medicine, Selcuk University, Konya, Turkey.
Discov Oncol. 2024 Jan 27;15(1):19. doi: 10.1007/s12672-024-00871-y.
There is limited data on the relationship of miRNAs with parameters that may affect surgical management or reflect tumour prognosis. It was aimed to evaluate serum miRNA levels in breast carcinoma cases and reveal the relationship between these levels and prognosis-related factors such as the histological type of the tumour, estrogen receptor, progesterone receptor, Ki-67 index, HER-2neu, E-cadherin, tumour size, CK5/6, CA15.3 levels, number of tumour foci, number of metastatic lymph nodes, and status of receiving neoadjuvant therapy. Thirty-five patients with a histopathologically confirmed breast carcinoma diagnosis in the case group and 35 healthy individuals in the control group were examined. miR-206, miR-17-5p, miR-125a, miR-125b, miR-200a, Let-7a, miR-34a, miR-31, miR-21, miR-155, miR-10b, miR-373, miR-520c, miR-210, miR-145, miR-139-5p, miR-195, miR-99a, miR-497 and miR-205 expression levels in the serum of participants were determined using the Polymerase Chain Reaction method. While serum miR-125b and Let-7a expression levels were significantly higher in breast cancer patients, miR-17-5p, miR-125a, miR-200a, miR-34a, miR-21, miR-99a and miR-497 levels were significantly lower in them. The Let-7a expression level had a statistically significant relationship with breast cancer histological type and HER-2neu parameters, miR-17-5p, miR-125b, Let-7a, miR-34a, miR-21 and miR-99a levels with E-cadherin, miR-34a, miR-99a and miR-497 with CA15.3, miR-125b, miR-200a and miR-34a with the number of metastatic lymph nodes, miR-125a with the number of tumour foci and miR-200a with the status of having the neoadjuvant therapy. Serum miR-17-5p, miR-125a, miR-125b, miR-200a, Let-7a, miR-34a, miR-21, miR-99a and miR-497 expression levels were determined to have predictive and prognostic importance in breast cancer.
关于微小RNA（miRNAs）与可能影响手术治疗或反映肿瘤预后的参数之间的关系，相关数据有限。本研究旨在评估乳腺癌患者血清中的miRNA水平，并揭示这些水平与预后相关因素之间的关系，这些因素包括肿瘤的组织学类型、雌激素受体、孕激素受体、Ki-67指数、HER-2neu、E-钙黏蛋白、肿瘤大小、CK5/6、CA15.3水平、肿瘤病灶数量、转移淋巴结数量以及接受新辅助治疗的情况。研究对病例组中35例经组织病理学确诊为乳腺癌的患者和对照组中35名健康个体进行了检查。采用聚合酶链反应法测定了参与者血清中miR-206、miR-17-5p、miR-125a、miR-125b、miR-200a、Let-7a、miR-34a、miR-31、miR-21、miR-155、miR-10b、miR-373、miR-520c、miR-210、miR-145、miR-139-5p、miR-195、miR-99a、miR-497和miR-205的表达水平。乳腺癌患者血清中miR-125b和Let-7a的表达水平显著更高，而miR-17-5p、miR-125a、miR-200a、miR-34a、miR-21、miR-99a和miR-497的水平则显著更低。Let-7a的表达水平与乳腺癌组织学类型和HER-2neu参数具有统计学显著关系，miR-17-5p、miR-125b、Let-7a、miR-34a、miR-21和miR-99a水平与E-钙黏蛋白有关，miR-34a、miR-99a和miR-497与CA15.3有关，miR-
