Institute for Hygiene and Microbiology, University of Würzburg, Germany; Department of Ophthalmology, University Hospital Würzburg, Germany.
Institute for Hygiene and Microbiology, University of Würzburg, Germany.
Int J Med Microbiol. 2024 Mar;314:151602. doi: 10.1016/j.ijmm.2024.151602. Epub 2024 Jan 23.
Fusarium keratitis is a severe infection of the anterior eye, frequently leading to keratoplasty or surgical removal of the affected eye. A major risk factor for infection is the use of contact lenses. Inadequate hygiene precautions and mold-growth permissive storage fluids are important risk factors for fungal keratitis. The aim of this study was to comparatively analyze contact lens storage fluids disinfection efficacy against Fusarium species.
Eleven commercially available storage fluids were tested. The storage fluids were classified according to their active ingredients myristamidopropyldimethylamine (Aldox), polyhexanide and hydrogen peroxide. Efficacy was tested against isolates belonging to the Fusarium solani and Fusarium oxysporum species complexes as the most common agents of mould keratitis. Tests were carried out based on DIN EN ISO 14729.
All Aldox and hydrogen peroxide (HO) based fluids were effective against Fusarium spp., while the majority of polyhexanide based storage fluids showed only limited or no antifungal effects. Efficacy of polyhexanide could be restored by the addition of the pH-regulating agent tromethamine - an additive component in one commercially available product.
In summary, the use of Aldox- or hydrogen peroxide-based storage fluids may reduce the risk of Fusarium keratitis, while polyhexanide-based agents largely lack efficacy against Fusarium.
镰刀菌角膜炎是一种严重的前眼部感染，常导致角膜移植或手术切除受影响的眼睛。感染的一个主要危险因素是使用隐形眼镜。卫生防护措施不足和霉菌生长允许的储存液是真菌角膜炎的重要危险因素。本研究旨在比较分析针对镰刀菌属的隐形眼镜储存液消毒效果。
测试了 11 种市售的储存液。根据其活性成分肉豆蔻酰丙基二甲基胺（Aldox）、聚己双胍和过氧化氢，将储存液进行分类。针对最常见的霉菌角膜炎病原体茄病镰刀菌和尖孢镰刀菌种复合体的分离株进行了功效测试。测试是根据 DIN EN ISO 14729 进行的。
所有 Aldox 和基于 HO 的液体对镰刀菌属均有效，而大多数基于聚己双胍的储存液仅显示有限或无抗真菌作用。通过添加 pH 调节剂三羟甲基氨基甲烷（一种市售产品的添加剂成分）可以恢复聚己双胍的功效。
综上所述，使用基于 Aldox 或过氧化氢的储存液可能会降低患镰刀菌角膜炎的风险，而基于聚己双胍的制剂对镰刀菌属的功效则大大降低。
