Hao Haibo, Yue Yihong, Chen Qun, Yang Yan, Kuai Benke, Wang Qian, Xiao Tingting, Chen Hui, Zhang Jinjing
National Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; State Key Laboratory of Genetic Engineering and Fudan Center for Genetic Diversity and Designing Agriculture, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China.
National Research Center for Edible Fungi Biotechnology and Engineering, Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Sci Total Environ. 2024 Mar 15;916:170226. doi: 10.1016/j.scitotenv.2024.170226. Epub 2024 Jan 26.
Cultivation of Stropharia rugosoannulata with straw in forestland is effective for straw biodegradation and can prevent the waste of straw resources and environmental pollution and generate economic benefits. However, there is a lack of systematic evaluation of spent mushroom substrate (SMS) input into forestland, such as soil properties and microbial succession. In this experiment, 0 (CK), 10 (SA), 20 (SB), 30 (SC), 40 (SD), and 50 (SE) kg/m straw were used to cultivate S. rugosoannulata, and two soil layers (0-10 cm, 10-20 cm) of the cultivated forestland were analyzed. The results indicated that SMS significantly promoted nutrient accumulation in forestland. The bacterial alpha diversity in the SC treatment group was greater than that in the control and gradually decreased to the control level with interannual changes, while the trend of fungal alpha diversity was opposite to that of bacterial alpha diversity. Furthermore, the SC treatment group positively affected soil nitrogen metabolism-related microorganisms for two consecutive years and significantly promoted tree growth. Habitat niche breadth and null model analysis revealed that bacterial communities were more sensitive than fungal communities after SMS input. Linear mixed model (LMM) analysis revealed that SMS supplementation significantly positively affected bacteria (Gammaproteobacteria and Bacteroidota) and significantly negatively affected fungi (Coniochaetales). The constructed fungal-bacterial co-occurrence networks exhibited modularity, and the five types of bacteria were significantly correlated with soil organic matter (SOM), soil organic carbon (SOC), available potassium (AK), available phosphorus (AAP) and available nitrogen (AN) levels. The structural equation model (SEM) showed that bacterial diversity responded more to changes in soil nutrients than did fungal diversity. Overall, 30 kg/m of straw decomposition and 2 years of continuous cultivation were beneficial to soil health. This study provides new insights into the rational decomposition of straw and maintenance of forestland ecological balance by S. rugosoannulata.
利用稻草在林地栽培皱环盖菇对稻草生物降解有效，可防止稻草资源浪费和环境污染，并产生经济效益。然而，对于废弃菌棒输入林地后，如土壤性质和微生物演替等方面，缺乏系统评价。本试验采用0（CK）、10（SA）、20（SB）、30（SC）、40（SD）和50（SE）kg/m稻草栽培皱环盖菇，并对栽培林地的两个土层（0-10厘米、10-20厘米）进行分析。结果表明，废弃菌棒显著促进了林地养分积累。SC处理组的细菌α多样性高于对照组，并随年份变化逐渐降至对照水平，而真菌α多样性的变化趋势与细菌α多样性相反。此外，SC处理组连续两年对土壤氮代谢相关微生物有积极影响，并显著促进树木生长。生境生态位宽度和零模型分析表明，废弃菌棒输入后细菌群落比真菌群落更敏感。线性混合模型（LMM）分析表明，添加废弃菌棒对细菌（γ-变形菌纲和拟杆菌门）有显著正向影响，对真菌（座囊菌目）有显著负向影响。构建的真菌-细菌共现网络具有模块性，5种细菌与土壤有机质（SOM）、土壤有机碳（SOC）、速效钾（AK）、速效磷（AAP）和速效氮（AN）水平显著相关。结构方程模型（SEM）表明，细菌多样性对土壤养分变化的响应比真菌多样性更敏感。总体而言，30 kg/m的稻草分解和连续2年的栽培有利于土壤健康。本研究为皱环盖菇合理分解稻草和维持林地生态平衡提供了新的见解。
