Xiang Wen, Yin Guoliang, Liu Haoming, Wei Jiayi, Yu Xinghui, Xie Yan, Zhang Li, Jiang Wentao, Lu Naiyan
School of Medicine, Nankai University, Tianjin, China; Department of Liver Transplantation, Tianjin First Central Hospital, Tianjin, China; Tianjin Key Laboratory of Molecular and Treatment of Liver Cancer, Tianjin First Center Hospital, Tianjin, China.
School of Food Science and Technology, Jiangnan University, Wuxi, China.
Int J Biol Macromol. 2024 Mar;261(Pt 1):129670. doi: 10.1016/j.ijbiomac.2024.129670. Epub 2024 Jan 26.
The oxidative microenvironment in fibrotic livers often diminishes the effectiveness of mesenchymal stem cells (MSCs)-based therapy. Recent research suggests that pharmacological pre-treatment could enhance the therapeutic performance of MSCs. In this study, we assessed the impact of Arctium lappa L. polysaccharides (ALP) on the biological properties of nasal ectomesenchymal stem cells (EMSCs) and investigated the augmenting effect of ALP pretreatment on EMSCs (ALP-EMSCs) for the treatment of liver fibrosis. ALP treatment demonstrated multiple biological impacts on EMSC functions regarding liver fibrosis: firstly, it maintained the stemness of the cells while boosting the EMSCs' paracrine effects; secondly, it increased the expression of anti-inflammatory and antioxidant factors; thirdly, it inhibited the activation of hepatic stellate cells (HSCs) and liver collagen build-up by modulating the Wnt/β-catenin signaling pathways. Collectively, these effects helped to halt the progression of liver fibrosis. Therefore, the use of ALP-EMSCs presents an innovative and promising approach for treating hepatic fibrosis in clinical scenarios.
纤维化肝脏中的氧化微环境常常会降低基于间充质干细胞（MSCs）的治疗效果。最近的研究表明，药物预处理可以提高MSCs的治疗性能。在本研究中，我们评估了牛蒡多糖（ALP）对鼻外胚间充质干细胞（EMSCs）生物学特性的影响，并研究了ALP预处理对EMSCs（ALP-EMSCs）治疗肝纤维化的增强作用。ALP处理对EMSCs在肝纤维化方面的功能产生了多种生物学影响：首先，它在增强EMSCs旁分泌作用的同时维持了细胞的干性；其次，它增加了抗炎和抗氧化因子的表达；第三，它通过调节Wnt/β-连环蛋白信号通路抑制肝星状细胞（HSCs）的激活和肝脏胶原蛋白的积累。总的来说，这些作用有助于阻止肝纤维化的进展。因此，使用ALP-EMSCs为临床治疗肝纤维化提供了一种创新且有前景的方法。
