Wang Yakun, Li Zufei, Lu Jun
Department of Pathology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Gongti South Road, Chaoyang District, Beijing, 100020, People's Republic of China.
Department of Otorhinolaryngology, Head and Neck Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People's Republic of China.
Sci Rep. 2024 Jan 27;14(1):2270. doi: 10.1038/s41598-024-52341-8.
Chronic rhinosinusitis with nasal polyps (CRSwNP) is a chronic inflammatory disease of the nasal mucosa, and epithelial-mesenchymal transition (EMT) is thought to be an essential process in the pathogenesis of CRSwNP. However, the mechanisms of epithelial and fibroblastic changes at the single-cell level are unclear. In this study, we investigated the epithelial cell, fibroblast, and key gene alterations in the development of CRSwNP. We revealed major cell types involved in CRSwNP and nasal mucosal inflammation formation, then mapped epithelial and fibroblast subpopulations. We showed that the apical and glandular epithelial cells and the ADGRB3+ and POSTN+ fibroblasts were the key cell subtypes in the progression of CRSwNP. Pseudotime and cell cycle analysis identified dynamic changes between epithelial cells and fibroblasts during its development. WFDC2 and CCL26 were identified as the key marker genes involved in the development of CRSwNP and were validated by IHC staining, which may provide a potential novel target for future CRSwNP therapy. ScRNA-seq data provided insights into the cellular landscape and the relationship between epithelial cells and fibroblasts in the progression of CRSwNP. WFDC2 and CCL26 were identified as the key genes involved in the development of CRSwNP and may be the potential markers for gene therapy.
伴鼻息肉的慢性鼻-鼻窦炎（CRSwNP）是一种鼻黏膜慢性炎症性疾病，上皮-间质转化（EMT）被认为是CRSwNP发病机制中的一个重要过程。然而，单细胞水平上皮细胞和成纤维细胞变化的机制尚不清楚。在本研究中，我们调查了CRSwNP发展过程中的上皮细胞、成纤维细胞和关键基因改变。我们揭示了参与CRSwNP和鼻黏膜炎症形成的主要细胞类型，然后绘制了上皮细胞和成纤维细胞亚群图谱。我们表明，顶端和腺上皮细胞以及ADGRB3+和成纤维细胞POSTN+是CRSwNP进展中的关键细胞亚型。伪时间和细胞周期分析确定了上皮细胞和成纤维细胞在其发育过程中的动态变化。WFDC2和CCL26被确定为参与CRSwNP发展的关键标记基因，并通过免疫组化染色进行了验证，这可能为未来CRSwNP治疗提供潜在的新靶点。单细胞RNA测序（ScRNA-seq）数据为CRSwNP进展过程中的细胞景观以及上皮细胞和成纤维细胞之间的关系提供了见解。WFDC2和CCL26被确定为参与CRSwNP发展的关键基因，可能是基因治疗的潜在标志物。
