Ostrowska-Podhorodecka Zofia, McCulloch Christopher A
Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada.
Wound Repair Regen. 2021 Jul;29(4):602-612. doi: 10.1111/wrr.12920. Epub 2021 Apr 22.
The intermediate filament protein vimentin is a widely used phenotypic marker for identifying cells of the mesenchymal linkage such as fibroblasts and myofibroblasts, but the full repertoire of vimentin's functional attributes has not been fully explored. Here we consider how vimentin, in addition to its contributions to mechanical stabilization of cell structure, also helps to control the assembly of cell adhesions and migration through collagen matrices. While the assembly and function of matrix adhesions are critical for the differentiation of myofibroblasts and many other types of adherent cells, a potential mechanism that explains how vimentin affects the recruitment and abundance of centrally important proteins in cell adhesions has been elusive. Here we review recent data indicating that vimentin plays a central regulatory role in the assembly of focal adhesions which form in response to the attachment to collagen. We show that in particular, vimentin is a key organizer of the β1 integrin adhesive machinery, which affects cell migration through collagen. This review provides a comprehensive picture of the surprisingly broad array of processes and molecules with which vimentin interacts to affect cell function in the context of fibroblast and myofibroblast adhesion and migration on collagen.
中间丝蛋白波形蛋白是一种广泛用于识别间充质连接细胞（如成纤维细胞和平滑肌成纤维细胞）的表型标志物，但波形蛋白功能特性的全部内容尚未得到充分探索。在这里，我们探讨了波形蛋白除了对细胞结构的机械稳定性有贡献外，还如何通过胶原基质帮助控制细胞黏附的组装和迁移。虽然基质黏附的组装和功能对于平滑肌成纤维细胞和许多其他类型的黏附细胞的分化至关重要，但解释波形蛋白如何影响细胞黏附中核心重要蛋白的募集和丰度的潜在机制一直难以捉摸。在这里，我们回顾了最近的数据，这些数据表明波形蛋白在响应与胶原的附着而形成的黏着斑组装中起核心调节作用。我们特别表明，波形蛋白是β1整合素黏附机制的关键组织者，它通过胶原影响细胞迁移。这篇综述全面介绍了波形蛋白在成纤维细胞和平滑肌成纤维细胞在胶原上的黏附及迁移过程中，与影响细胞功能的一系列令人惊讶的广泛过程和分子相互作用的情况。
