Department of Genetics and Reproductive Biotechnologies, Central European Institute of Technology - Veterinary Research Institute, Brno, Czech Republic.
Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Torino, Italy.
Int J Environ Health Res. 2024 Oct;34(10):3503-3514. doi: 10.1080/09603123.2024.2310152. Epub 2024 Jan 28.
Environmental exposure is associated with increased incidence of respiratory and cardiovascular diseases and reduced fertility. Exposure to air pollution can influence gene expression through epigenetic mechanisms. In this study, we analysed gene-specific CpG methylation in spermatozoa of city policemen occupationally exposed to air pollution in two Czech cities differing by sources and composition of the air pollution. In Prague, the pollution is mainly formed by NO2 from heavy traffic. Ostrava is a hotspot of industrial air pollution with high concentrations of particular matter (PM) and benzo[a]pyrene (B[a]P). We performed genome-wide methylation sequencing using the SureSelectXT Human Methyl-Seq system (Agilent Technologies) and next-generation sequencing to reveal differentially methylated CpG sites and regions. We identified differential methylation in the region chr5:662169 - 663376 annotated to genes CEP72 and TPPP. The region was then analysed in sperm DNA from 117 policemen using targeted methylation sequencing, which proved its hypermethylation in sperm of Ostrava policemen.
环境暴露与呼吸道和心血管疾病发病率的增加以及生育能力的降低有关。暴露于空气污染会通过表观遗传机制影响基因表达。在这项研究中，我们分析了在捷克的两个城市中职业暴露于空气污染的城市警察的精子中特定基因的 CpG 甲基化情况，这两个城市的空气污染来源和成分不同。在布拉格，污染主要由来自交通繁忙的二氧化氮形成。俄斯特拉发是工业空气污染的热点地区，其颗粒物（PM）和苯并[a]芘（B[a]P）浓度很高。我们使用 SureSelectXT Human Methyl-Seq 系统（Agilent Technologies）和下一代测序进行了全基因组甲基化测序，以揭示差异甲基化的 CpG 位点和区域。我们在注释到 CEP72 和 TPPP 基因的 chr5:662169-663376 区域中发现了差异甲基化。然后，我们使用靶向甲基化测序分析了来自 117 名警察的精子 DNA，证明了在俄斯特拉发警察的精子中该区域发生了过度甲基化。
