光生物调节对HaCaT角质形成细胞产生的炎性细胞因子的影响。

Effect of photobiomodulation on inflammatory cytokines produced by HaCaT keratinocytes.

作者信息

Sá Maria Gabriela Rolim Silveira, Queiroz-Junior Celso Martins, Souza Paulo Eduardo Alencar de, Diniz Ivana Márcia Alves, Oliveira Maria Clara Moreira, Grossmann Soraya de Mattos Camargo, Souto Giovanna Ribeiro

机构信息

Postgraduate Program in Dentistry, Department of Dentistry, Pontifical Catholic University of Minas Gerais, Belo Horizonte, Brazil.

Laboratório Avançado em Saúde e Estudos Regenerativos - LASER | Biotecnologias.

出版信息

J Oral Biol Craniofac Res. 2024 Jan-Feb;14(1):79-85. doi: 10.1016/j.jobcr.2023.12.007. Epub 2024 Jan 8.

DOI:10.1016/j.jobcr.2023.12.007

PMID:38282697

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10819743/

Abstract

OBJECTIVE

to evaluate the effects of the red and near-infrared wavelength lasers in isolated and simultaneous way on the modulation of inflammatory cytokines produced by human keratinocytes (HaCaT) challenged by cytokines of human monocytes stimulated by lipopolysaccharide from .

DESIGN

HaCaT cells was previously exposed to the laser with wavelengths red (660 nm), near-infrared (808 nm). Then, HaCat cells were stimulated with the supernatant of lipopolysaccharide-challenged peripheral blood cells. The cytokines expressed by HaCat cells were measured using multiplex CBA assay.

RESULTS

HaCaT cells increased the production of inflammatory cytokines when stimulated with infrared laser compared to the control group (IFN-α2, IFN-γ, TNF-α, MCP-1, IL-6, IL-8, IL-10, IL -12p70, IL -17A, IL-23, IL-33), the red laser group (IFN-γ and IL-23) and the group of two lasers used simultaneously (IFN-α2, IFN-γ, IL-6 and IL-8, IL-17A, IL-18 and IL-23) (p < 0.05). The red laser also stimulated an increase in the expression of IFN-α2 by HaCaT cells in relation to the control group (p < 0.05).

CONCLUSION

Infrared laser, with an energy density of 5 J/cm, appear to be able to modulate inflammatory cytokines produced by HaCaT cells challenged by human monocyte cytokines.

摘要

目的

评估红色和近红外波长激光单独及同时作用对人角质形成细胞（HaCaT）产生的炎性细胞因子的调节作用，这些细胞因子由脂多糖刺激的人单核细胞的细胞因子所激发。

设计

将HaCaT细胞预先暴露于波长为红色（660nm）、近红外（808nm）的激光下。然后，用脂多糖刺激的外周血细胞的上清液刺激HaCaT细胞。使用多重CBA分析测定HaCaT细胞表达的细胞因子。

结果

与对照组（IFN-α2、IFN-γ、TNF-α、MCP-1、IL-6、IL-8、IL-10、IL-12p70、IL-17A、IL-23、IL-33）、红色激光组（IFN-γ和IL-23）以及同时使用两种激光的组（IFN-α2、IFN-γ、IL-6和IL-8、IL-17A、IL-18和IL-23）相比，HaCaT细胞在受到红外激光刺激时炎性细胞因子的产生增加（p<0.05）。与对照组相比，红色激光也刺激HaCaT细胞中IFN-α2的表达增加（p<0.05）。