Schell R F, Babu J P, Le Frock J L
Am J Clin Pathol. 1979 Aug;72(2):199-203. doi: 10.1093/ajcp/72.2.199.
Selection of an anaerobic blood culture based upon clinical findings that have compared the isolation rates of bacteremic agents from different blood culture media. No agreement has been reached as to which of the commercially available blood culture media is optimal for detection of bacteremia. The purpose of this study was to determine the rates of recovery of anaerobic microorganisms from various anaerobic blood culture media. The blood culture media were inoculated with a small inoculum of microorganisms in the presence or absence of an erythrocyte-serum mixture. The results demonstrated that the type of medium and the erythrocyte-serum mixture influenced the ability of blood culture media to support the growth of microorganisms. The majority of the media failed to support the growth of 87% or more of the microorganisms within four days after inoculation. Pre-reduced brain-heart infusion broth supported the growth of a larger proportion of microorganisms than the other types of blood culture media.
基于临床发现选择厌氧血培养,这些临床发现比较了不同血培养基中菌血症病原体的分离率。关于哪种市售血培养基最适合检测菌血症,尚未达成共识。本研究的目的是确定从各种厌氧血培养基中回收厌氧微生物的比率。在有或没有红细胞 - 血清混合物的情况下,用少量微生物接种物接种血培养基。结果表明,培养基类型和红细胞 - 血清混合物会影响血培养基支持微生物生长的能力。大多数培养基在接种后四天内无法支持87%或更多微生物的生长。预还原脑心浸液肉汤比其他类型的血培养基能支持更大比例微生物的生长。