脑β1-微管蛋白中两个半胱氨酸的位置，在去除可交换的鸟苷三磷酸后可发生交联。
Location of two cysteines in brain beta 1-tubulin that can be cross-linked after removal of exchangeable GTP.
作者信息
Little M, Ludueña R F
出版信息
Biochim Biophys Acta. 1987 Mar 18;912(1):28-33. doi: 10.1016/0167-4838(87)90243-3.
PMID:3828353
Abstract
Two cysteines of the major neuronal beta-tubulin subunit (beta 1) can be specifically cross-linked with the bifunctional sulfhydryl reagent N',N'-ethylenebis(iodoacetamide) after removal of GTP. A cysteine in position 12 cross-links with one of the cysteines in position 201 or 211. Although the two cross-linked cysteines are separated by at least 189 residues in the primary structure of tubulin, they are maximally 9 A apart in the tertiary structure.
摘要
去除鸟苷三磷酸（GTP）后，主要神经元β-微管蛋白亚基（β1）的两个半胱氨酸可与双功能巯基试剂N'，N'-亚乙基双（碘乙酰胺）特异性交联。第12位的一个半胱氨酸与第201位或211位的一个半胱氨酸交联。尽管在微管蛋白一级结构中，这两个交联的半胱氨酸被至少189个残基隔开，但在三级结构中它们相距最大为9埃。
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