Xu Yinglin, Gong Ye, Li Songlin, Zhou Yue, Ma Zhixiao, Yi Ganfeng, Chen Naisong, Wang Weilong, Huang Xuxiong
Key Laboratory of Agriculture Ministry for Freshwater Aquatic Genetic Research, Shanghai Ocean University, Shanghai 201306, China.
China-ASEAN "The Belt and Road" Joint Laboratory of Mariculture Technology, Ministry of Science and Technology of China, Shanghai Ocean University, Shanghai 201306, China.
Aquac Nutr. 2024 Jan 19;2024:9944159. doi: 10.1155/2024/9944159. eCollection 2024.
The present study explored the effects of inositol on growth performance, body composition, antioxidant performance, and lipid metabolism of largemouth bass (). Six isonitrogenous and isolipidic diets containing 0 mg/kg (G1, control), 125 mg/kg (G2), 250 mg/kg (G3), 375 mg/kg (G4), 500 mg/kg (G5), and 625 mg/kg (G6) inositol were prepared and fed to cultured fish (initial weight: 110 ± 1 g) for 8 weeks in recirculating the aquaculture systems. The results indicated that compared with G1 group, the weight gain rate (WGR), specific growth rate (SGR), and feed efficiency rate (FER) in the G3 group were significantly higher. The crude lipid content of the whole fish and the liver of cultured fish was significantly reduced with increasing dietary inositol inclusion. However, no significant effects on moisture, crude protein, and ash contents of fish were observed among the different groups. Dietary inositol supplementation significantly increased muscular crude protein. However, muscular total lipid contents were decreased when the inclusion level was higher than 250 mg/kg (G3-G6 groups). As dietary inositol supplemental level increased, serum triglyceride (TG), and cholesterol (TC) contents showed an increasing trend and reached the maximum value in the G3 group. Additionally, serum low-density lipoprotein cholesterol (LDL-C) in G2, G3, G4, and G5 groups was significantly upregulated by increasing inositol. While, there was no significant change in serum high-density lipoprotein cholesterol (HDL-C) among the treatments. Inositol inclusion also significantly reduced the serum alkaline phosphatase (AKP), glutamic-pyruvic transaminase (ALT), and glutamic-oxaloacetic transaminase (AST) activities as well as serum malondialdehyde (MDA) content but significantly increased serum catalase (CAT), superoxide dismutase (SOD) activities, and total antioxidant capacity (T-AOC). Compared with the control group, the activities of hepatic total lipase (TL) and lipoprotein lipase (LPL) were significantly elevated in the G3, G4, and G5 groups. Above all, dietary inositol supplementation could improve growth performance and antioxidant capacity, and reduce the liver fat content of largemouth bass, and the optimal supplementation level of inositol in feed is estimated to be 250.31-267.27 mg/kg.
本研究探讨了肌醇对大口黑鲈生长性能、体成分、抗氧化性能和脂质代谢的影响。制备了六种等氮等脂日粮，分别含有0 mg/kg（G1，对照组）、125 mg/kg（G2）、250 mg/kg（G3）、375 mg/kg（G4）、500 mg/kg（G5）和625 mg/kg（G6）的肌醇，并在循环水养殖系统中投喂养殖鱼（初始体重：110 ± 1 g）8周。结果表明，与G1组相比，G3组的增重率（WGR）、特定生长率（SGR）和饲料效率率（FER）显著更高。随着日粮中肌醇添加量的增加，养殖鱼全鱼和肝脏的粗脂肪含量显著降低。然而，不同组之间未观察到对鱼体水分、粗蛋白和灰分含量的显著影响。日粮中添加肌醇显著增加了肌肉粗蛋白含量。然而，当添加水平高于250 mg/kg时（G3 - G6组），肌肉总脂质含量降低。随着日粮中肌醇添加水平的增加，血清甘油三酯（TG）和胆固醇（TC）含量呈上升趋势，并在G3组达到最大值。此外，G2、G3、G4和G5组中血清低密度脂蛋白胆固醇（LDL - C）随着肌醇添加量的增加而显著上调。同时，各处理组之间血清高密度脂蛋白胆固醇（HDL - C）无显著变化。添加肌醇还显著降低了血清碱性磷酸酶（AKP）、谷丙转氨酶（ALT）和谷草转氨酶（AST）活性以及血清丙二醛（MDA）含量，但显著增加了血清过氧化氢酶（CAT）、超氧化物歧化酶（SOD）活性和总抗氧化能力（T - AOC）。与对照组相比，G3、G4和G5组肝脏总脂肪酶（TL）和脂蛋白脂肪酶（LPL）的活性显著升高。综上所述，日粮中添加肌醇可以提高大口黑鲈的生长性能和抗氧化能力，降低肝脏脂肪含量，饲料中肌醇的最佳添加水平估计为250.31 - 267.27 mg/kg。
