Du Binbin, Wu Jindong, Wang Meng, Wu Jia, Sun Chaoyue, Zhang Xingen, Ren Xifeng, Wang Qifei
College of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu'an, Anhui, China.
Xingtai Agriculture and Rural Bureau, Xingtai, Hebei, China.
Front Plant Sci. 2024 Jan 11;14:1319889. doi: 10.3389/fpls.2023.1319889. eCollection 2023.
Improving barley grain quality is a major goal in barley breeding. In this study, a total of 35 papers focusing on quantitative trait loci (QTLs) mapping for barley quality traits published since 2000 were collected. Among the 454 QTLs identified in these studies, 349 of them were mapped onto high-density consensus maps, which were used for QTL meta-analysis. Through QTL meta-analysis, the initial QTLs were integrated into 41 meta-QTLs (MQTLs) with an average confidence interval (CI) of 1. 66 cM, which is 88.9% narrower than that of the initial QTLs. Among the 41 identified MQTLs, 25 were subsequently validated in publications using genome-wide association study (GWAS). From these 25 validated MQTLs, ten breeder's MQTLs were selected. Synteny analysis comparing barley and wheat MQTLs revealed orthologous relationships between eight breeder's MQTLs and 45 wheat MQTLs. Additionally, 17 barley homologs associated with rice quality traits were identified within the regions of the breeder's MQTLs through comparative analysis. The findings of this study provide valuable insights for molecular marker-assisted breeding and the identification of candidate genes related to quality traits in barley.
提高大麦籽粒品质是大麦育种的主要目标。在本研究中，共收集了自2000年以来发表的35篇聚焦于大麦品质性状数量性状位点（QTL）定位的论文。在这些研究中鉴定出的454个QTL中，有349个被定位到高密度共识图谱上，用于QTL元分析。通过QTL元分析，初始QTL被整合为41个元QTL（MQTL），平均置信区间（CI）为1.66 cM，比初始QTL窄88.9%。在鉴定出的41个MQTL中，有25个随后在使用全基因组关联研究（GWAS）的出版物中得到验证。从这25个经过验证的MQTL中，选出了10个育种者MQTL。大麦和小麦MQTL的共线性分析揭示了8个育种者MQTL与45个小麦MQTL之间的直系同源关系。此外，通过比较分析，在育种者MQTL区域内鉴定出17个与水稻品质性状相关的大麦同源基因。本研究结果为大麦分子标记辅助育种和品质性状相关候选基因的鉴定提供了有价值的见解。
