Department of Plant Production, Collage of Agriculture Science and Natural Resource, Gonbad Kavous University, Gonbad-e Kavus 4971799151, Iran.
Horticulture-Crops Reseaech Department, Golestan Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Gorgan 4969186951, Iran.
Genes (Basel). 2022 Nov 10;13(11):2087. doi: 10.3390/genes13112087.
Abiotic stresses cause a significant decrease in productivity and growth in agricultural products, especially barley. Breeding has been considered to create resistance against abiotic stresses. Pyramiding genes for tolerance to abiotic stresses through selection based on molecular markers connected to Mega MQTLs of abiotic tolerance can be one of the ways to reach Golden Barley. In this study, 1162 original QTLs controlling 116 traits tolerant to abiotic stresses were gathered from previous research and mapped from various populations. A consensus genetic map was made, including AFLP, SSR, RFLP, RAPD, SAP, DArT, EST, CAPS, STS, RGA, IFLP, and SNP markers based on two genetic linkage maps and 26 individual linkage maps. Individual genetic maps were created by integrating individual QTL studies into the pre-consensus map. The consensus map covered a total length of 2124.43 cM with an average distance of 0.25 cM between markers. In this study, 585 QTLs and 191 effective genes related to tolerance to abiotic stresses were identified in MQTLs. The most overlapping QTLs related to tolerance to abiotic stresses were observed in MQTL6.3. Furthermore, three MegaMQTL were identified, which explained more than 30% of the phenotypic variation. MQTLs, candidate genes, and linked molecular markers identified are essential in barley breeding and breeding programs to develop produce cultivars resistant to abiotic stresses.
非生物胁迫会导致农产品，特别是大麦的生产力和生长显著下降。通过基于与非生物耐受的 Mega MQTL 相关的分子标记的选择来培育对非生物胁迫的耐受性基因，可以是实现“黄金大麦”的方法之一。在这项研究中，从先前的研究中收集了 1162 个控制非生物胁迫耐受性的原始 QTL，并从各种群体中进行了图谱绘制。根据两个遗传连锁图谱和 26 个个体连锁图谱，利用 AFLP、SSR、RFLP、RAPD、SAP、DArT、EST、CAPS、STS、RGA、IFLP 和 SNP 标记制作了共识遗传图谱。通过将个体 QTL 研究整合到预共识图谱中，创建了个体遗传图谱。共识图谱总长度为 2124.43cM，标记之间的平均距离为 0.25cM。在这项研究中，在 MQTL 中鉴定了 585 个与非生物胁迫耐受性相关的 QTL 和 191 个有效基因。与非生物胁迫耐受性最相关的重叠 QTL 出现在 MQTL6.3 中。此外，还鉴定了三个 MegaMQTL，它们解释了超过 30%的表型变异。MQTL、候选基因和连锁的分子标记在大麦的育种和育种计划中是必不可少的，有助于开发具有非生物胁迫抗性的生产品种。
