Anna E J
Am J Med Technol. 1979 Jun;45(6):585-8.
Four agar media (blood, corn meal, chocolate, and Sabouraud's) and four liquid media (brain-heart infusion broth, one percent peptone broth, pooled human serum, and lyophilized rabbit coagulase plasma) were evaluated for their ability to enhance the production of capsules from four strains of Cryptococcus yeast within 48 hours at 25C and 35C. The yeast in serum and plasma was washed with distilled water prior to India ink preparation in order to prevent precipitation of the ink. The rabbit coagulase plasma was the only medium which consistently produced large capsules in 48 hours. Incubation temperatures and anticoagulant made little difference.
评估了四种琼脂培养基（血琼脂、玉米粉琼脂、巧克力琼脂和沙保弱琼脂）和四种液体培养基（脑心浸液肉汤、1%蛋白胨肉汤、混合人血清和冻干兔凝固酶血浆）在25℃和35℃下48小时内增强四株隐球菌酵母产生荚膜的能力。在制备印度墨汁之前，用蒸馏水洗涤血清和血浆中的酵母，以防止墨汁沉淀。兔凝固酶血浆是唯一能在48小时内持续产生大荚膜的培养基。培养温度和抗凝剂影响不大。
