Khodarev N N, Sokolova I A, Aleksandrova S S, Volgina V V, Votrin I I
Biull Eksp Biol Med. 1987 Mar;103(3):312-4.
Highly purified preparations of Ca/Mg-dependent cell nuclear endonuclease have been obtained from human spleen lymphocytes. The purification was 2000-fold, and the enzyme was a polypeptide with a molecular weight of 57-54 kD. The study of its binding with monoclonal antibody enzyme, produced by various hybridoma strains obtained upon the immunization of mice with non-fractionated nuclear extract has shown that Ca/Mg-dependent endonuclease was the best expressed endonuclease of lymphocyte cellular nuclei. Possible production of some other cell nuclear endonucleases during processing of CH/Mg-dependent endonuclease is suggested.
已从人脾淋巴细胞中获得了高度纯化的钙/镁依赖性细胞核核酸内切酶制剂。纯化倍数为2000倍，该酶是一种分子量为57 - 54 kD的多肽。用未分级的核提取物免疫小鼠后获得的各种杂交瘤菌株产生的单克隆抗体酶对其结合情况的研究表明，钙/镁依赖性核酸内切酶是淋巴细胞细胞核中表达最好的核酸内切酶。提示在钙/镁依赖性核酸内切酶的加工过程中可能产生一些其他的细胞核核酸内切酶。
