Department of Organic Agriculture Management, Faculty of Applied Sciences, Igdir University, Igdir, Turkey.
Research Laboratory Application and Research Center (ALUM), Igdir University, Igdir, Turkey.
Methods Mol Biol. 2024;2753:403-407. doi: 10.1007/978-1-0716-3625-1_24.
Zebrafish embryos are an important organism used as an in vivo model in a wide variety of disciplines from the past to the present. Immunohistochemistry analyses are an important method used to determine the localization of specific antigens in tissue sections with labeled antibodies depending on antigen-antibody interactions in zebrafish embryos. Immunofluorescence assays are an immunohistochemistry method that uses fluorophores to determine diverse cellular antigens. Zebrafish embryos and larvae, with their small size, are the most ideal model organisms for whole-mount immunohistochemical and immunofluorescent methods today. The small size of these organisms allows simultaneous evaluation of different tissues and organs, and results are obtained in a shorter time. In this section, whole-mount immunohistochemical and immunofluorescent analysis methods in zebrafish embryos, and larvae are summarized in detail, taking into account different studies and recent advances.
斑马鱼胚胎是一种重要的生物体，从过去到现在，它被广泛应用于各种学科的活体模型。免疫组织化学分析是一种重要的方法，用于通过标记抗体与抗原-抗体相互作用，在组织切片中确定特定抗原的定位。免疫荧光测定是一种免疫组织化学方法，它使用荧光团来确定各种细胞抗原。斑马鱼胚胎和幼虫由于其体积小，是目前最理想的全胚胎免疫组织化学和免疫荧光方法的模式生物。这些生物体的体积小，可以同时评估不同的组织和器官，并且可以在更短的时间内获得结果。在这一部分中，详细总结了斑马鱼胚胎和幼虫的全胚胎免疫组织化学和免疫荧光分析方法，考虑到了不同的研究和最新进展。
