利用纤维素层析法从植物中分离 dsRNA。

Isolation of dsRNA from Plants by Cellulose Chromatography.

机构信息

College of Plant Protection, Northeast Agricultural University, Harbin, Heilongjiang, China.

出版信息

Methods Mol Biol. 2024;2771:13-17. doi: 10.1007/978-1-0716-3702-9_3.

Abstract

As the constitutive molecules of double-stranded RNA (dsRNA) virus genomes and replicative intermediates of single-stranded RNA (ssRNA) viruses, the high-molecular-weight dsRNAs are commonly found in RNA virus-infected plants. Therefore, the dsRNA is recognized as a hallmark of RNA virus infection and the profile of dsRNA has been applied as an efficient tool for diagnoses or characterization of unreported RNA viruses. Cellulose chromatography is one of the most useful procedures for the isolation of viral dsRNAs from total nucleic acids. Here, we describe rapid cellulose-based methods for purification of dsRNAs from plant tissue.

摘要

作为双链 RNA（dsRNA）病毒基因组的组成分子和单链 RNA（ssRNA）病毒的复制中间体，高分子量 dsRNA 通常存在于感染 RNA 病毒的植物中。因此，dsRNA 被认为是 RNA 病毒感染的标志，dsRNA 的图谱已被应用于诊断或鉴定未报告的 RNA 病毒。纤维素色谱是从总核酸中分离病毒 dsRNA 的最有用程序之一。在这里，我们描述了一种从植物组织中快速纯化 dsRNA 的基于纤维素的方法。