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植物病毒的即时诊断:当前趋势与前景

Point of care diagnosis of plant virus: Current trends and prospects.

作者信息

Kalimuthu Kalishwaralal, Arivalagan Jaison, Mohan Manikandan, Samuel Selvan Christyraj Johnson Retnaraj, Arockiaraj Jesu, Muthusamy Ramakrishnan, Ju Ho-Jong

机构信息

Department of Agricultural Biology, College of Agriculture & Life Sciences, Jeonbuk National University, Jeonju-si, 54896, Republic of Korea; Division of Cancer Research, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, 695014, Kerala, India.

Department of Chemistry, Molecular Biosciences and Proteomics Center of Excellence, Northwestern University, Evanston, IL, 60208, USA.

出版信息

Mol Cell Probes. 2022 Feb;61:101779. doi: 10.1016/j.mcp.2021.101779. Epub 2021 Nov 16.

Abstract

Plant viral diseases accounts for major global economic losses in modern-day agriculture. Plant viral disease management is the primary challenge for both farmers and researchers. Detection and identification of plant viruses are of paramount importance for successful management of a viral disease. Recent advancements in molecular biology have contributed to significant progress in the development of new, sensitive, and effective diagnostic methods. However, most techniques are neither time/cost-effective nor user-friendly and require sophisticated labs. Hence, the past few decades of agricultural research have mainly focused on developing farmer-friendly, point-of-care diagnostic tools that provide high-sensitive rapid diagnosis. The current trend in plant virus diagnostic tools is cheaper, easy-to-use portable devices with no compromise on sensitivity and reproducibility.

摘要

植物病毒病在现代农业中造成了重大的全球经济损失。植物病毒病的管理是农民和研究人员面临的主要挑战。植物病毒的检测和鉴定对于成功管理病毒病至关重要。分子生物学的最新进展推动了新型、灵敏且有效的诊断方法的显著发展。然而,大多数技术既不节省时间/成本,也不便于用户使用,并且需要先进的实验室。因此,过去几十年的农业研究主要集中在开发对农民友好的即时诊断工具,以提供高灵敏度的快速诊断。当前植物病毒诊断工具的趋势是价格更便宜、易于使用的便携式设备,同时不牺牲灵敏度和可重复性。

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