Palucha Natálie, Fojt Jakub, Holátko Jiri, Hammerschmiedt Tereza, Kintl Antonin, Brtnický Martin, Řezáčová Veronika, De Winterb Karel, Uitterhaegen Evelien, Kučerík Jiří
Brno University of Technology, Institute of Chemistry and Technology of Environmental Protection, Purkyňova 118, Brno, 612 00, Czech Republic; Bio Base Europe Pilot Plant VZW, Rodenhuizekaai 1, Desteldonk, Gent, 9042, Belgium.
Textile Testing Institute, Cejl 480/12, 602 00, Brno, Czech Republic.
Chemosphere. 2024 Mar;352:141300. doi: 10.1016/j.chemosphere.2024.141300. Epub 2024 Jan 27.
The search for eco-friendly substitutes for traditional plastics has led to the production of biodegradable bioplastics. However, concerns have been raised about the impact of bioplastic biodegradation on soil health. Despite these concerns, the potential negative consequences of bioplastics during various stages of biodegradation remain underexplored. Therefore, this study aims to investigate the impact of micro-bioplastics made of poly-3-hydroxybutyrate (P3HB) on the properties of three different soils. In our ten-month experiment, we investigated the impact of poly-3-hydroxybutyrate (P3HB) on Chernozem, Cambisol, and Phaeozem soils. Our study focused on changes in soil organic matter (SOM), microbial activity, and the level of soil carbon and nitrogen. The observed changes indicated an excessive level of biodegradation of SOM after the soils were enriched with micro-particles of P3HB, with concentrations ranging from 0.1% to 3%. The thermogravimetric analysis confirmed the presence of residual P3HB (particularly in the 3% treatment) and underscored the heightened biodegradation of SOM, especially in the more stable SOM fractions. This was notably evident in Phaeozem soils, where even the stable SOM pool was affected. Elemental analysis revealed changes in soil organic carbon content following P3HB degradation, although nitrogen levels remained constant. Enzymatic activity was found to vary with soil type and responded differently across P3HB concentration levels. Our findings confirmed that P3HB acts as a bioavailable carbon source. Its biodegradation stimulates the production of enzymes, which in turn affects various soil elements, indicating complex interactions within the soil ecosystem.
对传统塑料的环保替代品的探索导致了可生物降解生物塑料的生产。然而，人们对生物塑料生物降解对土壤健康的影响提出了担忧。尽管存在这些担忧，但生物塑料在生物降解各个阶段的潜在负面后果仍未得到充分探索。因此，本研究旨在调查由聚-3-羟基丁酸酯（P3HB）制成的微塑料对三种不同土壤性质的影响。在我们为期十个月的实验中，我们研究了聚-3-羟基丁酸酯（P3HB）对黑钙土、始成土和灰化土的影响。我们的研究重点是土壤有机质（SOM）、微生物活性以及土壤碳氮水平的变化。观察到的变化表明，在土壤中添加浓度为0.1%至3%的P3HB微粒后，SOM的生物降解水平过高。热重分析证实了残留P3HB的存在（特别是在3%的处理中），并强调了SOM生物降解的加剧，尤其是在更稳定的SOM组分中。这在灰化土中尤为明显，即使是稳定的SOM库也受到了影响。元素分析表明，P3HB降解后土壤有机碳含量发生了变化，尽管氮水平保持不变。酶活性随土壤类型而变化，并且在不同的P3HB浓度水平下有不同的反应。我们的研究结果证实，P3HB作为一种生物可利用的碳源。其生物降解刺激了酶的产生，进而影响各种土壤元素，表明土壤生态系统中存在复杂的相互作用。
