Programa de Doctorado en Ciencias Biomédicas, Facultad de Ciencias de la Salud, Grupo Infección e Inmunidad - Universidad Tecnológica de Pereira, Pereira 660003, Colombia; Grupo de Investigaciones Herpetológicas y Toxinológicas, Centro de Investigaciones Biomédicas-Bioterio, Departamento de Biología, Facultad de Ciencias Naturales, Exactas y de la Educación, Universidad del Cauca, Popayán 190002, Colombia.
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, UNAM. Apartado Postal 510-3, Cuernavaca, Morelos 61500, Mexico.
Acta Trop. 2024 Apr;252:107134. doi: 10.1016/j.actatropica.2024.107134. Epub 2024 Jan 28.
The venom fractions of three buthid scorpion species from Colombia, C. margaritatus, T. pachyurus and T. n. sp. aff. metuendus, were examined for antimicrobial and toxicity toward mice and insects. The three venoms were separated into individual fractions using RP-HPLC, resulting in 85 fractions from C. margaritatus, 106 from T. pachyurus, and 70 from T. n. sp. aff. metuendus. The major fractions from the three scorpion venoms, which were eluted between 35 and 50 min, were tested for antimicrobial activity and toxicity. It was confirmed that the venom of the three species contains fractions with antimicrobial peptides that were evaluated against two bacterial strains of public health importance, Pseudomonas aeruginosa and Staphylococcus aureus. The venom of C. margaritatus had two antimicrobial fractions that showed activity against the named tested strains. The venom of T. pachyurus had three fractions that showed activity against S. aureus and two against both bacterial strains. Finally, the venom of T. n. sp. aff. metuendus had one fraction that showed activity against S. aureus, and five fractions showed activity against both bacterial strains. Also, some peptide fractions from the three venoms were toxic to mice. Last, the venoms of C. margaritatus and T. pachyurus were used as immunogens to obtain neutralizing antibodies against its respective venoms and to observe antibody recognition to related and unrelated scorpion venoms. A total of 15 mg of lyophilized antibodies were able to neutralize 1.5⋅LD of the venoms from T. n. sp. aff. metuendus, T. pachyurus and C. margaritatus, respectively. This information provides valuable insights into the diversity of each species' venom and their potential role in antimicrobial and venom toxicity.
从哥伦比亚的三种蝎子物种（C. margaritatus、T. pachyurus 和 T. n. sp. aff. metuendus）的毒液中分离出抗菌和对小鼠和昆虫毒性的活性组分。使用 RP-HPLC 将三种毒液分离成单独的组分，得到 C. margaritatus 的 85 个组分、T. pachyurus 的 106 个组分和 T. n. sp. aff. metuendus 的 70 个组分。从三种蝎子毒液中洗脱时间在 35 到 50 分钟之间的主要组分进行了抗菌活性和毒性测试。证实这三种毒液都含有抗菌肽的组分，对两种具有公共卫生重要性的细菌菌株（铜绿假单胞菌和金黄色葡萄球菌）进行了评估。C. margaritatus 的毒液有两个抗菌组分对上述测试菌株表现出活性。T. pachyurus 的毒液有三个对抗金黄色葡萄球菌的活性组分，两个对抗两种细菌的活性组分。最后，T. n. sp. aff. metuendus 的毒液有一个对抗金黄色葡萄球菌的活性组分，有五个对抗两种细菌的活性组分。此外，三种毒液的一些肽组分对小鼠有毒性。最后，使用 C. margaritatus 和 T. pachyurus 的毒液作为免疫原，获得针对各自毒液的中和抗体，并观察抗体对相关和不相关蝎子毒液的识别。总共 15 毫克冻干抗体能够中和 T. n. sp. aff. metuendus、T. pachyurus 和 C. margaritatus 毒液的 1.5 ⋅LD。这些信息为每个物种毒液的多样性及其在抗菌和毒液毒性方面的潜在作用提供了有价值的见解。
