Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province, College of Pharmacy, Qinghai Nationalities University, Xining, China.
Department of Pharmacy, West China Hospital, Sichuan University, Chengdu, China.
Luminescence. 2024 Jan;39(1):e4671. doi: 10.1002/bio.4671.
Copper is a critical element in both human and animal metabolic processes. Its role includes supporting connective tissue cross-linking, as well as iron and lipid metabolism; at the same time, copper is also a toxic heavy metal that can cause harm to both the environment and human health. Glutathione (GSH) is a tripeptide composed of glutamic acid, cysteine, and glycine combined with sulfhydryl groups. Its properties include acting as an antioxidant and facilitating integrative detoxification. GSH is present in both plant and animal cells and has a fundamental role in maintaining living organisms. GSH is the most abundant thiol antioxidant in the human body. It exists in reduced and oxidized forms within cells and provides significant biochemical functions, such as regulating vitamins such as vitamins D, E, and C, and facilitating detoxification. A fluorescent probe has been developed to detect copper ions selectively, sensitively, and rapidly. This report outlines the successful work on creating a peptide probe, TGN (TPE-Trp-Pro-Gly-Cln-His-NH ), with specific Cu detection capabilities, and a significant fluorescence recovery occurred with the addition of GSH. This indicates that the probe can detect Cu and GSH concurrently. The detection limit for Cu in the buffer solution was 264 nM (R = 0.9992), and the detection limit for GSH using the TGN-Cu complex was 919 nM (R = 0.9917). The probe exhibits high cell permeability and low biotoxicity that make it ideal for live cell imaging in biological conditions. This peptide probe has the capability to detect Cu and GSH in biological cells.
铜是人类和动物代谢过程中的关键元素。它的作用包括支持结缔组织交联，以及铁和脂质代谢；同时，铜也是一种有毒的重金属，会对环境和人类健康造成危害。谷胱甘肽（GSH）是一种由谷氨酸、半胱氨酸和甘氨酸结合巯基组成的三肽。其性质包括作为抗氧化剂和促进综合解毒。GSH 存在于植物和动物细胞中，在维持生物体内起着基本作用。GSH 是人体中最丰富的硫醇抗氧化剂。它以还原和氧化形式存在于细胞中，并提供重要的生化功能，如调节维生素 D、E 和 C 等维生素的功能，并促进解毒。已经开发出一种荧光探针来选择性、灵敏地和快速地检测铜离子。本报告概述了成功开发具有特定 Cu 检测能力的肽探针 TGN（TPE-Trp-Pro-Gly-Cln-His-NH ）的工作，并且随着 GSH 的加入，发生了显著的荧光恢复。这表明该探针可以同时检测 Cu 和 GSH。在缓冲溶液中，Cu 的检测限为 264 nM（R = 0.9992），使用 TGN-Cu 配合物检测 GSH 的检测限为 919 nM（R = 0.9917）。该探针具有高细胞通透性和低生物毒性，使其成为生物条件下活细胞成像的理想选择。这种肽探针能够检测生物细胞中的 Cu 和 GSH。
