College of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumqi, P. R. China.
College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi, P. R. China.
Luminescence. 2024 Jan;39(1):e4680. doi: 10.1002/bio.4680.
As an oxidant, deodorant and bleaching agent, the hypochlorous acid (HClO) and hypochlorite (ClO ) are widely used in corrosion inhibitors, textile dyes, pharmaceutical intermediates and in our daily lives. However, excess usage or aberrant accumulation of ClO leads to tissue damage or some diseases and even cancer. Therefore, it is necessary to develop a fluorescent probe that specifically identifies ClO . In this article, we synthesized a deep-red xanthene-based fluorescent probe (XA-CN). The strong electron deficient group dicyano endows the probe XA-CN deep-red fluorescent emission with high solubility, selectivity and sensitivity for ClO detection. Studies showed that the probe demonstrated turn-off fluorescence (643 nm) at the presence of ClO in dimethylsulfoxide/phosphate-buffered saline 1:1 (pH 9) solution with a limit of detection of 1.64 μM. Detection mechanism investigation revealed that the electron deficient group -CN and the hydroxyl group was oxidized into aldehyde or carbonyl groups at the presence of ClO , resulting ultraviolet-visible absorption of the probe blue shifted and turned-off fluorescence. Furthermore, XA-CN was successfully used for the detection of ClO in tap water samples.
次氯酸（HClO）和次氯酸盐（ClO ）作为氧化剂、除臭剂和漂白剂，广泛应用于缓蚀剂、纺织染料、医药中间体以及我们的日常生活中。然而，ClO 的过量使用或异常积累会导致组织损伤或某些疾病，甚至癌症。因此，有必要开发一种专门识别 ClO 的荧光探针。本文合成了一种基于深红光香豆素的荧光探针（XA-CN）。强缺电子基团二氰基赋予探针 XA-CN 对 ClO 检测具有高溶解度、选择性和灵敏度的深红光荧光发射。研究表明，探针在二甲亚砜/磷酸盐缓冲盐水 1:1（pH 9）溶液中存在 ClO 时表现出关闭荧光（643nm），检测限为 1.64μM。检测机制研究表明，在 ClO 的存在下，缺电子基团-CN 和羟基被氧化成醛或羰基，导致探针的紫外-可见吸收蓝移并关闭荧光。此外，XA-CN 成功用于自来水中 ClO 的检测。
