Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Shandong Kangqiao Biotechnology Co. Ltd, Binzhou 256500, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Oct 15;299:122830. doi: 10.1016/j.saa.2023.122830. Epub 2023 May 10.
The level of HClO/ClO in mitochondria is essential to keep the normal function of mitochondria. Therefore, it is meaningful to accurately and quickly monitor ClO in mitochondria. In this work, a new triphenylamine-based fluorescence probe PDTPA was designed and synthesized, in which pyridinium salt and dicyano-vinyl group were introduced as mitochondria targeting site and reaction site for ClO. The probe showed high sensitivity and fast fluorescence response (<10 s) in the detection of ClO. Moreover, the probe PDTPA had good linearity in a wide concentration range of ClO and its detection limit was calculated as 10.5 μM. Confocal fluorescence images demonstrated that the probe could target mitochondria and track the fluctuations of endogenous/exogenous ClO levels in the mitochondria of living cells.
线粒体中 HClO/ClO 的水平对于维持线粒体的正常功能至关重要。因此，准确、快速地监测线粒体中的 ClO 具有重要意义。在本工作中，设计并合成了一种基于三苯胺的新型荧光探针 PDTPA，其中引入了吡啶鎓盐和二氰基乙烯基作为线粒体靶向位点和 ClO 的反应位点。该探针在检测 ClO 时表现出高灵敏度和快速的荧光响应（<10s）。此外，探针 PDTPA 在 ClO 的宽浓度范围内具有良好的线性关系，其检测限计算为 10.5μM。共聚焦荧光图像表明，该探针可以靶向线粒体，并跟踪活细胞线粒体中内源性/外源性 ClO 水平的波动。
