测定来自伊朗西南部设拉子临床样本中产 ESBL 和碳青霉烯酶奇异变形杆菌的最小抑菌浓度（MIC）的银和氧化锌纳米粒子的细胞毒性。

Determining the cytotoxicity of the Minimum Inhibitory Concentration (MIC) of silver and zinc oxide nanoparticles in ESBL and carbapenemase producing Proteus mirabilis isolated from clinical samples in Shiraz, Southwest Iran.

机构信息

Department of Bacteriology and Virology, School of Medicine, Student Research Committee of Shiraz, University of Medical Sciences, Shiraz, Iran.

Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.

出版信息

BMC Res Notes. 2024 Jan 29;17(1):40. doi: 10.1186/s13104-023-06402-2.

DOI:10.1186/s13104-023-06402-2

PMID:38287416

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10826267/

Abstract

OBJECTIVE

Proteus mirabilis is related to serious infections. The present study was designed to investigate the minimum inhibitory concentration (MIC) of silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnONPs) and cytotoxicity among P. mirabilis isolates recovered from clinical samples in Shiraz.

RESULTS

A total of 100 P. mirabilis isolates were screened by biochemical tests and polymerase chain reaction (PCR). Also, 25 (25%) and 7 (7%) isolates were positive for extended-spectrum beta-lactamase (ESBLs) and carbapenemase, respectively. Synthesized nanoparticles were characterized by UV-vis spectrum, X-ray diffraction (XRD), and electron microscopy. The average size of AgNPs and ZnONPs in the present study is 48 and < 70 nm, respectively. The MIC and the MBC of the ZnONPs were in the range of 31.25 µg/ml and 62.5 µg/mL, respectively. Also, for AgNPs, the MIC and the MBC were in the range of 7.8 µg/mL and 15.6 µg/mL, respectively. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay in a primary culture of fibroblast L929 cells for this MIC indicated biocompatibility and low cytotoxicity of Ag NPs and for ZnONPs indicated significant cytotoxicity. Also, a MIC of AgNPs can be used as a therapeutic concentration without the effect of cytotoxicity in human cells.

摘要

目的

奇异变形杆菌与严重感染有关。本研究旨在调查从设拉子临床样本中分离出的奇异变形杆菌分离株的最小抑菌浓度（MIC）和银纳米粒子（AgNPs）和氧化锌纳米粒子（ZnONPs）的细胞毒性。

结果

通过生化试验和聚合酶链反应（PCR）对 100 株奇异变形杆菌进行了筛选。此外，25（25%）和 7（7%）株分别对超广谱β-内酰胺酶（ESBLs）和碳青霉烯酶呈阳性。合成的纳米粒子通过紫外可见光谱、X 射线衍射（XRD）和电子显微镜进行了表征。本研究中 AgNPs 和 ZnONPs 的平均粒径分别为 48nm 和<70nm。ZnONPs 的 MIC 和 MBC 分别在 31.25µg/ml 和 62.5µg/ml 范围内。此外，对于 AgNPs，MIC 和 MBC 分别在 7.8µg/ml 和 15.6µg/ml 范围内。MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴盐）测定法用于原代成纤维细胞 L929 细胞的该 MIC 表明 Ag NPs 具有生物相容性和低细胞毒性，而 ZnONPs 则表现出显著的细胞毒性。此外，AgNPs 的 MIC 可作为无细胞毒性的治疗浓度用于人类细胞。