Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.
Oral Dis. 2024 Oct;30(7):4740-4750. doi: 10.1111/odi.14858. Epub 2024 Jan 29.
This study proposed to assess the effect of Cryptocarya moschata extract on single and mixed biofilms formed on denture base and reline acrylic resin.
Single and mixed biofilms of Candida albicans and Streptococcus mutans were formed on the samples and treated with C. moschata extract; Nystatin solution at 100,000 IU/mL or Penicillin antibiotic solution at 100,000 IU/mL; or PBS solution. Antimicrobial activity was analyzed by counting colony-forming units, metabolism assay, assessment of protein components of the biofilm matrix, and of cell viability using confocal laser scanning microscopy (CLSM). Data were submitted to ANOVA and Tukey's post-test (α = 0.05).
Cryptocarya moschata extract reduced cell viability of C. albicans and S. mutans single and mixed biofilms formed on samples. For all types of biofilms in the C. moschata group, there was a log reduction of the biofilm, proven by the Alamar Blue assay. Analyzing the extracellular matrix protein components, groups treated with the extract exhibited a lower level of fluorescence compared to the PBS groups. Reduction in thickness biofilm and viable cells was perceptible in the C. moschata group when assessing through CLSM.
Cryptocarya moschata extract reduced the single and mixed biofilms of C. albicans and S. mutans on acrylic resins.
本研究旨在评估樟科植物提取物对义齿基托和义齿衬里丙烯酸树脂上单种和混合生物膜的影响。
在样本上形成白色念珠菌和变形链球菌的单种和混合生物膜，并分别用樟科植物提取物、浓度为 100000IU/mL 的制霉菌素溶液或浓度为 100000IU/mL 的青霉素抗生素溶液或 PBS 溶液处理。通过菌落形成单位计数、代谢测定、生物膜基质蛋白成分评估以及使用共聚焦激光扫描显微镜（CLSM）评估细胞活力来分析抗菌活性。数据采用方差分析和 Tukey 事后检验（α=0.05）。
樟科植物提取物降低了样本上单种和混合生物膜中白色念珠菌和变形链球菌的细胞活力。对于樟科植物组的所有类型生物膜，Alamar Blue 测定法均证明生物膜发生了对数减少。与 PBS 组相比，用提取物处理的各组细胞外基质蛋白成分的荧光水平较低。通过 CLSM 评估，在樟科植物组中可明显观察到生物膜厚度和活细胞减少。
樟科植物提取物可减少丙烯酸树脂上单种和混合白色念珠菌和变形链球菌生物膜的形成。
