Cryptocarya 属提取物的抗菌效果和生物相容性。
Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species.
机构信息
Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (Unesp), Araraquara, São Paulo, Brazil.
Department of Biochemistry and Organic Chemistry, Institute of Chemistry, São Paulo State University (Unesp), Araraquara, São Paulo, Brazil.
出版信息
PLoS One. 2021 Dec 31;16(12):e0261884. doi: 10.1371/journal.pone.0261884. eCollection 2021.
This study evaluated the efficacy of Cryptocarya spp extracts on biofilm of Candida albicans and its biocompatibility. Mature biofilm of C. albicans was formed on denture base acrylic resin samples and the fungicidal effect of the extracts was evaluated by Alamar Blue® assay, counting colony-forming units (CFU/mL) and confocal laser scanning microscopy (CLSM). Cytotoxicity of extracts from Cryptocarya species was evaluated by AlamarBlue® assay, using normal oral keratinocytes (NOK) cells. In additional, Analysis of plant extracts by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UPLC-DAD-MS) was performed. The results showed significant reduction in the cellular metabolism and in the number of CFU/mL of C. albicans (p<0.05). The concentration of 0.045 g/mL completely inhibited the number of CFU/mL. Regarding cytotoxicity, all extracts decreased cell viability compared to the control group. CLSM analysis showed predominance of live cells, but with a great difference between the groups. Antimicrobial activity of extracts from Cryptocarya on C. albicans biofilm was confirmed. However, all extracts showed toxicity on NOK cells.
本研究评估了Cryptocarya 属提取物对白色念珠菌生物膜的功效及其生物相容性。在义齿基托丙烯酸树脂样本上形成成熟的白色念珠菌生物膜，通过 Alamar Blue® assay 评估提取物的杀菌作用，计算菌落形成单位（CFU/mL）和共聚焦激光扫描显微镜（CLSM）。通过 AlamarBlue® assay 评估来自 Cryptocarya 种的提取物对正常口腔角质细胞（NOK）细胞的细胞毒性。此外，通过超高效液相色谱-二极管阵列检测器-串联质谱法（UPLC-DAD-MS）对植物提取物进行分析。结果表明，Cryptocarya 属提取物显著降低了细胞代谢和 CFU/mL 中白色念珠菌的数量（p<0.05）。浓度为 0.045 g/mL 完全抑制了 CFU/mL 的数量。关于细胞毒性，与对照组相比，所有提取物均降低了细胞活力。CLSM 分析显示活细胞占优势，但各组之间存在很大差异。证实了 Cryptocarya 属提取物对白色念珠菌生物膜的抗菌活性。然而，所有提取物对 NOK 细胞均显示出毒性。
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