Crutcher K A, Collins F
Brain Res. 1986 Dec 10;399(2):383-9. doi: 10.1016/0006-8993(86)91533-7.
Nerve growth factor (NGF) is present in high concentrations in the rat hippocampal formation where it may be involved in sympathetic sprouting following septohippocampal denervation. In addition, recent evidence suggests that some forebrain cholinergic neurons, including septohippocampal neurons, are responsive to exogenous NGF. Since septohippocampal neurons have been shown to sprout in response to entorhinal lesions both in rats and, recently, in humans, we sought to determine whether endogenous NGF-like activity increases in the rat hippocampal formation following injury to the entorhinal cortex. We found that entorhinal lesions which result in extensive denervation of the dentate granule cells, and subsequent sprouting of septohippocampal axons, do result in greater NGF-like growth-promoting activity in medium conditioned by slices of the denervated tissue when compared to medium conditioned by control tissue. These results suggest that brain NGF may be involved in injury-induced sprouting of forebrain cholinergic neurons.
神经生长因子（NGF）在大鼠海马结构中浓度很高，它可能参与隔海马去神经支配后的交感神经萌发。此外，最近的证据表明，一些前脑胆碱能神经元，包括隔海马神经元，对外源性NGF有反应。由于已经证明隔海马神经元在大鼠以及最近在人类中会因内嗅皮质损伤而萌发，我们试图确定内嗅皮质损伤后大鼠海马结构中内源性NGF样活性是否增加。我们发现，导致齿状颗粒细胞广泛去神经支配以及随后隔海马轴突发芽的内嗅皮质损伤，与对照组织条件培养基相比，去神经支配组织切片条件培养基中确实具有更高的NGF样促生长活性。这些结果表明，脑NGF可能参与损伤诱导的前脑胆碱能神经元萌发。
