Collins F, Crutcher K A
J Neurosci. 1985 Oct;5(10):2809-14. doi: 10.1523/JNEUROSCI.05-10-02809.1985.
Conditioned medium prepared from slices of the rat hippocampal formation contains an agent that shares the following properties with nerve growth factor (NGF): it promotes neurite growth from embryonic sympathetic ganglia in vitro; and it is inhibited by affinity-purified antibody against mouse submaxillary gland 2.5 S NGF. The NGF-like growth-promoting activity is regionally distributed within the hippocampal formation: the activity is consistently higher in the dentate gyrus-CA3 region than in the CA1 region. Furthermore, the level of activity is significantly increased within 1 week after a medial septal lesion, and, in the dentate-CA3 region, the increased level of activity is maintained for at least 4 weeks after the lesion. Control lesions that fail to interrupt the septohippocampal innervation, but cause equally extensive damage to nearby regions of the central nervous system, do not cause increased levels of activity in the hippocampal formation. These results provide substantial evidence linking the NGF-like agent in hippocampal conditioned medium to the sprouting of sympathetic axons into the dentate gyrus-CA3 region of the hippocampal formation after a medial septal lesion in vivo.
从大鼠海马结构切片制备的条件培养基中含有一种与神经生长因子（NGF）具有以下共同特性的因子：它在体外可促进胚胎交感神经节的神经突生长；并且它被针对小鼠颌下腺2.5S NGF的亲和纯化抗体所抑制。这种NGF样的促生长活性在海马结构内呈区域分布：齿状回-CA3区的活性始终高于CA1区。此外，内侧隔区损伤后1周内活性水平显著升高，并且在齿状回-CA3区，损伤后活性升高水平至少维持4周。未能中断隔海马神经支配但对中枢神经系统附近区域造成同等广泛损伤的对照损伤，不会导致海马结构中活性水平升高。这些结果提供了大量证据，将海马条件培养基中的NGF样因子与体内内侧隔区损伤后交感轴突向海马结构齿状回-CA3区的发芽联系起来。
