Lewis J, Westerberg V, Corcoran M E
Brain Res. 1987 Feb 17;403(2):205-12. doi: 10.1016/0006-8993(87)90057-6.
High-performance liquid chromatography with electrochemical detection was used to measure the regional concentrations of monoamines and metabolites in the brains of rats killed 2 or 4 weeks after kindling of generalized seizures with amygdaloid stimulation. Each kindled rat was compared to a yoked control that received brief trains of non-convulsive low-frequency stimulation of the amygdala. Two weeks after kindling we found a significant depletion of noradrenaline (NA) in the ipsilateral frontal cortex, a significant depletion of serotonin (5-HT) in the stimulated amygdala and contralateral hypothalamus, and no significant changes in concentration of dopamine (DA). Four weeks after kindling we found significant depletions of NA in the stimulated amygdala and ipsilateral hypothalamus, a significant depletion of 5-HT in the ipsilateral hippocampus, and no significant changes in DA. These findings generally fail to replicate previous reports of monoaminergic correlates of kindling. Furthermore, the alterations in monoamines produced by kindling do not fall into a simple and readily interpretable pattern.
采用高效液相色谱-电化学检测法，测定在用杏仁核刺激引发全身性癫痫发作2周或4周后处死的大鼠脑内单胺类物质及其代谢产物的局部浓度。将每只点燃大鼠与其配对的对照大鼠进行比较，对照大鼠接受杏仁核的短串非惊厥性低频刺激。点燃后2周，我们发现同侧额叶皮质去甲肾上腺素（NA）显著减少，受刺激的杏仁核和对侧下丘脑5-羟色胺（5-HT）显著减少，多巴胺（DA）浓度无显著变化。点燃后4周，我们发现受刺激的杏仁核和同侧下丘脑NA显著减少，同侧海马体5-HT显著减少，DA无显著变化。这些发现总体上未能重复先前关于点燃与单胺能相关性的报道。此外，点燃所产生的单胺类物质变化并不呈现出简单且易于解释的模式。
