Plant Quarantine Technology Center, Animal and Plant Quarantine Agency, Gimcheon, Korea.
Department of Plant Medicals, Andong National University, Andong, Korea.
Arch Insect Biochem Physiol. 2024 Jan;115(1):e22074. doi: 10.1002/arch.22074.
The fall armyworm (FAW), Spodoptera frugiperda, is one of the most harmful plant pests in the world and is globally distributed from the American continent to the Asian region. The FAW USA population (Sf-USA) and China population (Sf-CHN), which belong to corn strain, showed different developmental periods and fecundity rates in lab conditions. Sf-USA had faster development and higher fecundity compared with Sf-CHN. To examine these differences, transcriptomic data from two FAW populations were analyzed and compared. Twelve gigabytes of transcripts were read from each sample and 21,258 differentially expressed genes (DEGs) were detected. DEGs with log2 fold change ≥ 2 were identified and compared in two populations. In comparison to the Sf-CHN, we discovered that 3471 and 3851 individual DEGs upregulated and downregulated, respectively. Comparing transcriptome profiles for differential gene expression revealed several DEGs, including 39 of ecdysone (E)-, 25 of juvenile hormone-, and 15 of insulin-related genes. We selected six of E-related genes, such as Neverland, Shade, Ecdysone receptor, Ecdysone-inducible protein 74 (E74), E75, and E78 from DEGs. Gene expressions were suppressed by RNA interference to confirm the physiological functions of the selected genes from Sf-USA. The Sf-USA showed developmental retardation and a decrease in fecundity rate by suppression of E-related genes. These findings show that biological characteristics between Sf-USA and Sf-CHN are influenced by E-related genes.
草地贪夜蛾（FAW），也称秋黏虫，是世界上最具危害性的植物害虫之一，分布范围从美洲大陆到亚洲地区。FAW 的美国种群（Sf-USA）和中国种群（Sf-CHN）属于玉米品系，在实验室条件下表现出不同的发育时期和繁殖率。与 Sf-CHN 相比，Sf-USA 的发育速度更快，繁殖率更高。为了研究这些差异，我们对两个 FAW 种群的转录组数据进行了分析和比较。每个样本读取了 12 吉字节的转录本，共检测到 21258 个差异表达基因（DEGs）。比较两个种群中 log2 倍变化≥2 的 DEGs。与 Sf-CHN 相比，我们发现分别有 3471 和 3851 个个体的 DEGs 上调和下调。比较差异基因表达的转录组图谱，揭示了几个 DEGs，包括 39 个蜕皮激素（E）-、25 个保幼激素-和 15 个胰岛素相关基因。我们从 DEGs 中选择了 6 个 E 相关基因，如 Neverland、Shade、Ecdysone receptor、Ecdysone-inducible protein 74 (E74)、E75 和 E78。通过 RNA 干扰抑制基因表达，以确认 Sf-USA 中选择基因的生理功能。抑制 E 相关基因后，Sf-USA 的发育迟缓，繁殖率下降。这些发现表明 Sf-USA 和 Sf-CHN 之间的生物学特征受到 E 相关基因的影响。
