Iwoń Zuzanna, Krogulec Ewelina, Kierlańczyk Aleksandra, Baranowska Patrycja, Łopianiak Iwona, Wojasiński Michal, Jastrzębska Elżbieta
Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Warsaw, Poland.
Nencki Institute of Experimental Biology PAS, Warsaw, Poland.
Biomed Mater. 2024 Feb 12;19(2). doi: 10.1088/1748-605X/ad240a.
Currently, numerous studies are conducted using nanofibers as a scaffold for culture cardiac cells; however, there still needs to be more research evaluating the impact of the physicochemical properties of polymer nanofibers on the structure and function of cardiac cells. We have studied how poly(-caprolactone) and polyurethane nanofibrous mats with different physicochemical properties influence the viability, morphology, orientation, and maturation of cardiac cells. For this purpose, the cells taken from different species were used. They were rat ventricular cardiomyoblasts (H9c2), mouse atrial cardiomyocytes (CMs) (HL-1), and human ventricular CMs. Based on the results, it can be concluded that cardiac cells cultured on nanofibers exhibit greater maturity in terms of orientation, morphology, and gene expression levels compared to cells cultured on polystyrene plates. Additionally, the physicochemical properties of nanofibers affecting the functionality of cardiac cells from different species and different parts of the heart were evaluated. These studies can support research on understanding and explaining mechanisms leading to cellular maturity present in the heart and the selection of nanofibers that will effectively help the maturation of CMs.
目前，有许多研究使用纳米纤维作为培养心脏细胞的支架；然而，仍需要更多研究来评估聚合物纳米纤维的物理化学性质对心脏细胞结构和功能的影响。我们研究了具有不同物理化学性质的聚己内酯和聚氨酯纳米纤维垫如何影响心脏细胞的活力、形态、取向和成熟度。为此，使用了来自不同物种的细胞。它们是大鼠心室心肌母细胞（H9c2）、小鼠心房心肌细胞（CMs）（HL-1）和人类心室CMs。根据结果可以得出结论，与在聚苯乙烯平板上培养的细胞相比，在纳米纤维上培养的心脏细胞在取向、形态和基因表达水平方面表现出更高的成熟度。此外，还评估了纳米纤维的物理化学性质对来自不同物种和心脏不同部位的心脏细胞功能的影响。这些研究有助于理解和解释导致心脏细胞成熟的机制，并有助于选择能有效促进心肌细胞成熟的纳米纤维。
