Naito M, Kudo I, Mukai-Sato Y, Tsushima S, Nomura H, Nojima S, Inoue K
Cancer Immunol Immunother. 1987;24(2):158-64. doi: 10.1007/BF00205594.
Liposomes composed of chemically synthesized glyceroglycolipids, such as 1,2-dipalmityl-[beta-cellobiosyl-(1'----3)]- glycerol (Cel-DAG), 1,2-dipalmityl-[beta-lactosyl-(1'----3)]-glycerol, or 1,2-dipalmityl-[beta-maltosyl-(1'----3)]-glycerol, were found to enhance protective immunity against transplantable tumor cells (sarcoma 180) in ICR mice. Peritoneal exudate cells prepared from mice treated in vivo with Cel-DAG showed cytostatic activity in vitro against the mouse leukemia cell line, EL-4. Adherent cells separated from this preparation showed similar activity. Peritoneal cells from polypeptone-injected mice acquired appreciable cytostatic activity when incubated in vitro in the presence of glyceroglycolipid liposomes. The adherent cell fraction alone showed rather weak cytostatic activity when pretreated with the glyceroglycolipids, and full activity was restored by supplementing with the nonadherent cell fraction. The ability of glycolipids to induce tumoricidal effects was affected by cholesterol content: with increasing cholesterol content, the activities decreased. Cholesterol-free glycolipid liposomes were taken more efficiently by macrophages than cholesterol-containing liposomes. Cholesterol modifies the surface property of glyceroglycolipid liposomes. Activation of macrophages is responsible for enhancement of protective immunity against tumor cells by injection of these glycolipids in vivo.
由化学合成的甘油糖脂组成的脂质体，如1,2-二棕榈酰基-[β-纤维二糖基-(1'→3)]-甘油（Cel-DAG）、1,2-二棕榈酰基-[β-乳糖基-(1'→3)]-甘油或1,2-二棕榈酰基-[β-麦芽糖基-(1'→3)]-甘油，被发现可增强ICR小鼠对可移植肿瘤细胞（肉瘤180）的保护性免疫。用Cel-DAG进行体内处理的小鼠制备的腹腔渗出细胞在体外对小鼠白血病细胞系EL-4表现出细胞抑制活性。从该制剂中分离出的贴壁细胞表现出类似的活性。当在甘油糖脂脂质体存在下体外孵育时，来自注射了蛋白胨的小鼠的腹腔细胞获得了明显的细胞抑制活性。单独的贴壁细胞部分在用甘油糖脂预处理时表现出相当弱的细胞抑制活性，通过补充非贴壁细胞部分可恢复全部活性。糖脂诱导杀肿瘤作用的能力受胆固醇含量影响：随着胆固醇含量增加，活性降低。无胆固醇的糖脂脂质体比含胆固醇的脂质体更有效地被巨噬细胞摄取。胆固醇改变了甘油糖脂脂质体的表面性质。巨噬细胞的激活负责通过在体内注射这些糖脂来增强对肿瘤细胞的保护性免疫。
