Sunamoto J, Iwamoto K, Inoue K, Endo T, Nojima S
Biochim Biophys Acta. 1982 Mar 8;685(3):283-8. doi: 10.1016/0005-2736(82)90069-4.
To understand the role of omega-cyclohexyl fatty acid residue of lipids in acido-thermophilic bacterial membranes, three unusual phosphatidylcholines, 1, 2-di-11-cyclohexylundecanoyl-L-alpha-phosphatidylcholine (11CYPC), 1,2-di-13-cyclohexyltridecanoyl-L-alpha-phosphatidylcholine (13CYPc), and 1-13-cyclohexyltridecanoyl-2-11-cyclohexylundecanoyl-L-alpha- phosphatidylcholine (1-13CY-2-11CYPC) were prepared and the steady-state fluorescence anisotropy of 1, 6-diphenylhexatriene (DPH) in the hydrophobic domain of these liposomal bilayers was determined. Compared with the case of dipalmitoyl (DPPC) or dimyristoyl phosphatidylcholine (DMPC), introducing the omega-cyclohexyl moiety onto lecithins makes the bilayers fluid below the phase transition temperature, while immobilizes them above the phase transition temperatures. The properties of the unusual phosphatidylcholine liposomes suggested by the steady-state fluorescence anisotropy investigation were in good agreement with those obtained from the thermotropic and permeability investigations. Results obtained are discussed from the view point of the role and function of lipid membranes of acido-thermophilic bacteria which contain unusual fatty acids.
为了解脂质的ω-环己基脂肪酸残基在嗜酸嗜热菌细胞膜中的作用，制备了三种不同寻常的磷脂酰胆碱，即1,2-二-11-环己基十一烷酰-L-α-磷脂酰胆碱（11CYPC）、1,2-二-13-环己基十三烷酰-L-α-磷脂酰胆碱（13CYPc）和1-13-环己基十三烷酰-2-11-环己基十一烷酰-L-α-磷脂酰胆碱（1-13CY-2-11CYPC），并测定了这些脂质体双层疏水区域中1,6-二苯基己三烯（DPH）的稳态荧光各向异性。与二棕榈酰磷脂酰胆碱（DPPC）或二肉豆蔻酰磷脂酰胆碱（DMPC）的情况相比，在卵磷脂上引入ω-环己基部分会使双层在相变温度以下呈流体状态，而在相变温度以上则使其固定化。稳态荧光各向异性研究表明的不同寻常的磷脂酰胆碱脂质体的性质与热致性和渗透性研究得到的性质高度一致。从含有不寻常脂肪酸的嗜酸嗜热菌脂质膜的作用和功能的角度对所得结果进行了讨论。
