Iijima T, Kondo T, Hasegawa K
Cell Tissue Res. 1987 Jan;247(1):167-77. doi: 10.1007/BF00216559.
The innervation of the arteriovenous anastomoses in the dog tongue has been investigated. At the light-microscopic level, the vessels were found to be densely supplied with adrenergic and AChE-positive nerve plexuses and less densely with the quinacrine-binding nerve plexus. At the electron-microscopic level, at least two apparently different types of axon profiles were identified: Small vesicle-containing axons, characterized by many small granular vesicles, variable numbers of small clear vesicles and large granular vesicles. Storage of endogenous amines and uptake of exogenous amines into most small granular vesicles and many large granular vesicles was demonstrated. These axons stained only lightly with reaction products for AChE activity and thus seemed to be adrenergic in nature. Some axons contained numerous large granular vesicles, whose cores occasionally stained with uranyl ions; this suggests a co-localization of ATP or peptides as neurotransmitters. Small granular vesicle-free axons, containing small clear vesicles and large granular vesicles in variable ratio. Most cores of these large granular vesicles were heavily stained with uranyl ions. No storage or uptake of amine into the synaptic vesicles was detected. Some axons appeared to be typically cholinergic, some, typically non-adrenergic, non-cholinergic, and the rest, intermediate between the two. All axons stained heavily with reaction products for AChE activity, suggesting their cholinergic nature.
对犬舌动静脉吻合处的神经支配进行了研究。在光镜水平，发现这些血管密集分布有肾上腺素能和乙酰胆碱酯酶（AChE）阳性神经丛，而奎纳克林结合神经丛分布较少。在电镜水平，至少鉴定出两种明显不同类型的轴突形态：含小泡的轴突，其特征为有许多小颗粒小泡、数量不等的小清亮小泡和大颗粒小泡。已证实内源性胺类物质储存在大多数小颗粒小泡和许多大颗粒小泡中，且外源性胺类物质被摄取到这些小泡中。这些轴突仅被AChE活性反应产物轻度染色，因此在本质上似乎是肾上腺素能的。一些轴突含有大量大颗粒小泡，其核心偶尔被铀离子染色；这表明ATP或肽类作为神经递质存在共定位现象。不含小颗粒小泡的轴突，含有比例不等的小清亮小泡和大颗粒小泡。这些大颗粒小泡的大多数核心被铀离子重度染色。未检测到胺类物质储存在突触小泡中或被摄取到突触小泡中。一些轴突似乎典型地为胆碱能，一些典型地为非肾上腺素能、非胆碱能，其余的则介于两者之间。所有轴突均被AChE活性反应产物重度染色，提示其胆碱能本质。
